Anti-HLA E antibody [MEM-E/02] (ab2216)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-HLA E antibody [MEM-E/02](ab2216)

IHC image of ab2216 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2216, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  Immunohistochemistry (Frozen sections) - HLA E antibody [MEM-E/02] (ab2216)

ab2216 staining HLA E in Human brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was permeabilized with methanol + 1% H₂O₂. Samples were incubated with primary antibody (1/100 in 4% serum + 1% BSA + 0.01% azide) for 12 hours. A Biotin-conjugated Horse anti-mouse IgG monoclonal (1/100) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Pascal Durrenberger

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