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Product Name
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HLA E antibody [MEM-E/02]
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See all HLA E antibodies (5)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [MEM-E/02] to HLA E
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Immunogen
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Recombinant denatured heavy chain (Human).
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Reacts with
(species key)
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Hu
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Specificity
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This antibody reacts with the denaturated heavy chain of human HLA-E.
It does not cross-react with HLA-A, -B, -C or -G.
Specifity of the antibody was confirmed on HLA-G/HLA-E Workshop(Victoria 2002).
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at an assay dependant dilution.
WB: 1/500-1/1000 (see Abreviews). Predicted molecular weight: 43 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Membrane; Single-pass type I membrane protein
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Research areas
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Immunology >> Adaptive Immunity >> MHC >> Class I
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Relevance
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HLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.
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HLA E antibody [MEM-E/02] (ab2216) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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MEM-E/02
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Isotype
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IgG1
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Purity
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>95% by SDS-PAGE
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from ascities by protein-A affinity chromatography.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HLA E antibody [MEM-E/02] is on this datasheet. But please do contact us if you would like any reassurance! |
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References for HLA E antibody [MEM-E/02] (ab2216)
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This product has been used in: (two most recent references)
Grotzke JE et al. The Mycobacterium tuberculosis phagosome is a HLA-I processing competent organelle. PLoS Pathog 5:e1000374 (2009). WB; Human. PubMed: 19360129
Gtherstrm C et al. Difference in gene expression between human fetal liver and adult bone marrow mesenchymal stem cells. Haematologica 90:1017-26 (2005). PubMed: 16079100
See all 3 publication references for this product.
If you publish research using ab2216 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to HLA E
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HLA E antibody [MEM-E/02] - more information
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Customer reviews (feedback) regarding HLA E antibody [MEM-E/02] |
Customer FAQs regarding HLA E antibody [MEM-E/02] |
Protocols for HLA E antibody [MEM-E/02] |
Price and availability of products related to HLA E antibody [MEM-E/02] |
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HLA E antibody [MEM-E/02] - related products:
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HLA E antibody [MEM-E/02] - other tools:
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Contact Abcam with a Technical Enquiry about HLA E antibody [MEM-E/02] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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