|
|
|
|
|
Product Name
|
HLA E antibody [MEM-E/06]
|
| |
See all HLA E antibodies (5)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [MEM-E/06] to HLA E
|
|
Immunogen
|
Bacterially expressed recombinant full length protein refolded with beta2-microglobulin and peptide (Human).
|
|
Reacts with
(species key)
|
Hu
|
|
Specificity
|
The antibody recognized native surface-expressed HLA E, but not denaturated heavy chain of HLA E. The published results showed that the antibody cross-reacts with some classical MHC Class I molecules (HLA A3, A11, B7). However, the recent Workshop I Session on the 3rd International Conference on HLA-G (Paris, July 2003) confirmed that the antibody exhibits much broader cross-reactivity classical MHC Class I antigens, namely with HLA A24, A32, B8, B15, B27, B35, B44, B54, C3, C4, C5 and C7.
|
|
Tested applications
(see key)
|
Flow Cyt, IP
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
Flow Cyt: Use at an assay dependant concentration.
IP: Use at an assay dependant concentration.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Membrane; Single-pass type I membrane protein
|
|
Research areas
|
Immunology >> Adaptive Immunity >> MHC >> Class I
|
|
Relevance
|
HLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
MEM-E/06
|
|
Isotype
|
IgG1
|
|
Purity
|
>95% by SDS-PAGE
|
|
Storage buffer
|
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Purification notes
|
Purified from ascites by protein A affinity chromatography.
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HLA E antibody [MEM-E/06] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
References for HLA E antibody [MEM-E/06] (ab3984)
|
|
This product has been used in:
Menier C et al. Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol 64:315-26 (2003). PubMed: 12590976
If you publish research using ab3984 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to HLA E
|
| |
HLA E antibody [MEM-E/06] - more information
|
Customer reviews (feedback) regarding HLA E antibody [MEM-E/06] |
Customer FAQs regarding HLA E antibody [MEM-E/06] |
Protocols for HLA E antibody [MEM-E/06] |
Price and availability of products related to HLA E antibody [MEM-E/06] |
| |
HLA E antibody [MEM-E/06] - related products:
|
|
|
| |
HLA E antibody [MEM-E/06] - other tools:
|
Contact Abcam with a Technical Enquiry about HLA E antibody [MEM-E/06] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
