Anti-HLA E antibody [MEM-E/07] (ab11820)

  Flow Cytometry - HLA E antibody [MEM-E/07] (ab11820)

Cells were treated with 500 Units of human IFN-gamma on 1 million ECV304 cells after 36 hours cells were harvested (PVG 0.1% trypsin), fixed with 2% paraformaldehyde and surface staining of HLA E was performed using ab11820 at a 1/100 dilution. The secondary used was a goat anti-mouse-FITC, at a 1/100 dilution.

Red line indicates the untreated expression level and black indicate the induced surface expression of HLA E. Gating strategy - total population.

Image courtesy of Shwetank Verma by Abreview.

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  Western blot - HLA E antibody [MEM-E/07] (ab11820)

HLA-G– or HLA-E–expressing HeLa cells were transfected with HCMV endoplasmic reticulum resident transmembrane glycoprotein US10. The lysates were immunoblotted with antibodies against HLA-G, HLA-E (ab11820), or beta-actin as a loading control. The figure shows that expression of HLA-G is slightly reduced by US10.

Image from Park B et al, J Exp Med. 2010 Aug 30;207(9):2033-41. Epub 2010 Aug 16, Fig S1. DOI 10.1084/jem.20091793