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Anti-HLA E antibody [MEM-E/08] (ab11821)

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Overview

Product name

Anti-HLA E antibody [MEM-E/08]
See all HLA E products (8) ...

Description

Mouse monoclonal [MEM-E/08] to HLA E

Specificity

The antibody recognized native surface-expressed HLA E, but not denaturated heavy chain of HLA E. The published results revealed that antibody is remarkably specific for HLA E, only with weak cross-reactivity with following classical MHC Class I molecules: HLA A24, B7, B27, B51, B54, C7.

Tested applications

WB, Flow Cyt, IPmore details

Cross reactivity

Reacts with

Human

Immunogen

Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein A purified

Purification notes

Purified from hybridoma culture supernatant by protein A-affinity chromatography.

Clonality

Monoclonal

Clone number

MEM-E/08

Isotype

IgG1

Research areas

Immunology >> Adaptive Immunity >> MHC >> Class I

  • Flow Cytometry - HLA E antibody [MEM-E/08] (ab11821)Flow Cytometry - HLA E antibody [MEM-E/08] (ab11821) image (enlarge)

  • Western blot - HLA E antibody [MEM-E/08] (ab11821)Western blot - HLA E antibody [MEM-E/08] (ab11821) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab11821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

HLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.

Cellular localization

Membrane; Single-pass type I membrane protein

Alternative names

  • HLA class I histocompatibility antigen E alpha chain antibody
  • DKFZp686P19218 antibody
  • EA1.2 antibody
  • EA2.1 antibody
  • HLA 6.2 antibody
  • HLA class I histocompatibility antigen alpha chain E antibody
  • HLA class I histocompatibility antigen E alpha chain precursor antibody
  • HLA-6.2 antibody
  • HLA6.2 antibody
  • HLAE antibody
  • Lymphocyte antigen antibody
  • Major histocompatibility complex class I E antibody
  • MHC antibody
  • MHC class I antigen E antibody
  • MHC HLA E alpha 1 antibody
  • MHC HLA E alpha 2.1 antibody
  • QA1 antibody
see all

Database links

Anti-HLA E antibody [MEM-E/08] images:

  Flow Cytometry - HLA E antibody [MEM-E/08] (ab11821)

Flow Cytometry - HLA E antibody [MEM-E/08] (ab11821)

ab11821 staining HLA E in Human K562 cells by Flow Cytometry. The sample was incubated with the primary antibody, (1/50), for 1 hour at 24°C. Ab6785(1/100) was used as secondary antibody.
Gating Strategy: Isotrope control

This image is courtesy of an anonymous Abreview

See Abreview

  Western blot - HLA E antibody [MEM-E/08] (ab11821)

Western blot - HLA E antibody [MEM-E/08] (ab11821)

Anti-HLA E antibody [MEM-E/08] (ab11821) at 1 µg/ml + Spleen (Human) Tissue Lysate - adult normal tissue (ab29699) at 10 µg

Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 40 kDa
Observed band size : 40 kDa
Additional bands at : 50 kDa,68 kDa,73 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 8 minutes

References for Anti-HLA E antibody [MEM-E/08] (ab11821)

This product has been referenced in:

  • Kalkunte SSet al. Vascular endothelial growth factor C facilitates immune tolerance and endovascular activity of human uterine NK cells at the maternal-fetal interface. J Immunol 182:4085-92 (2009). Flow Cyt; Human.Read more (PubMed: 19299706) »
  • Tennakoon DKet al. Therapeutic induction of regulatory, cytotoxic CD8+ T cells in multiple sclerosis. J Immunol 176:7119-29 (2006). Flow Cyt; Human.Read more (PubMed: 16709875) »

See all 3 publications for this product

Publishing research using ab11821? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"