Anti-HMG4 antibody (ab72544)
Key features and details
- Rabbit polyclonal to HMG4
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HMG4 antibody
See all HMG4 primary antibodies -
Description
Rabbit polyclonal to HMG4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to a region between residues 125 and 175 of human HMG4 (NP_005333.2).
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Positive control
- HeLa and 293T whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/2000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/2000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Binds preferentially single-stranded DNA and unwinds double stranded DNA. -
Tissue specificity
Expressed predominantly in placenta. -
Sequence similarities
Belongs to the HMGB family.
Contains 2 HMG box DNA-binding domains. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 3149 Human
- Entrez Gene: 15354 Mouse
- Entrez Gene: 305373 Rat
- Omim: 300193 Human
- SwissProt: O15347 Human
- SwissProt: O54879 Mouse
- Unigene: 19114 Human
- Unigene: 336087 Mouse
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Alternative names
- chromosomal protein, Nonhistone, HMG4 antibody
- High mobility group (nonhistone chromosomal) protein 4 antibody
- High mobility group box 3 antibody
see all
Images
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All lanes : Anti-HMG4 antibody (ab72544) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 23 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling HMG4 with ab72544 at 1/200 (1µg/ml). Detection: DAB.
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Detection of Human HMG4 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72544 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab72544 has not yet been referenced specifically in any publications.