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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab41878? Please let us know so that we can cite the reference in this datasheet
ab41878 has been referenced in 3 publications.
- Chen PS et al. miR-107 promotes tumor progression by targeting the let-7 microRNA in mice and humans. J Clin Invest 121:3442-55 (2011). WB; Mouse. PubMed: 21841313
- Watanabe M et al. HMGA-targeted phosphorothioate DNA aptamers increase sensitivity to gemcitabine chemotherapy in human pancreatic cancer cell lines. Cancer Lett : (2011). WB; Human. PubMed: 22036895
- Mori M et al. Zyxin mediates actin fiber reorganization in epithelial-mesenchymal transition and contributes to endocardial morphogenesis. Mol Biol Cell 20:3115-24 (2009). WB; Mouse. PubMed: 19439447
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - HMGA2 antibody (ab41878)
Anti-HMGA2 antibody (ab41878) at 1 µg/ml + MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 12 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
Additional bands at : 42 kDa,74 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
HMGA2 contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Immunoprecipitation - Anti-HMGA2 antibody (ab41878)
HMGA2 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to HMGA2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab41878.
Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
Band: 17kDa; HMGA2: 55kDa; Heavy Chains: Non Specific - 45 and 130kDa: We are unsure as to the identity of this extra band.
Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody (ab41878)
ICC/IF image of ab41878 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41878, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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