Anti-HMGA2 antibody (ab41878)

Reported problem with ab41878 not seeing band from human colorectal samples.

ANSWER:

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. To this end could you please provide a lot number or order number.

Having reviewed this case, I would like to offer some suggestions to help optimise the results from ab41878. I would also appreciate if you can confirm some further details:

-you say that the gel was examined by Coomassie blue staining after transfer, was the protein present?

You mention that both the blocking step and incubation with the secondary antibody was performed at 37°C. Whilst this may have been validated for the CST antibody we would not recommend it with the abcam antibody. I would suggest this be reduced to room temperature at most. Additionally I would attempt the primary antibody incubation at room temperature for 1 hour to see if this improves the results. It may also be worthwhile to try an alternative blocking agent such as BSA.

Should the suggestions not improve the results, please do let me know. In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

Dear officer,

I have been using the HMGA2 antibody ab41878 for a long time. It works very well. But I have a question whether you have tested the crossactivity with HMGA1a/b. And whhich part of the C-terminal region do you use to generate the antibody? Thanks a lot.

Kind Regards,

ANSWER:

Thank you for your inquiry and your positive feed back.

Unfortunately, the exact immunogen sequence is proprietary information.

But I am happy to inform you about the homology of the immunogen sequence and the different isoforms of HMGA1:

HMGA1a (P17096-1): 56%

HMGA1b (P17096-2): 56%

HMGA1c (P17096-3): 57%

We do not assume this antibody will cross react with any HMGA1 isoform due to the very low homology in the immunogen sequence.

But I have to confirm that this was not experimentally tested.

I hope this information is helpful. Please do not hesitate to contact me with any further question.