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Products:Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
Overview
Product name
Anti-HMGA2 antibody
See all HMGA2 products (5) ...
Description
Rabbit polyclonal to HMGA2
Tested applications
ICC/IF, IP, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide corresponding to C-terminal region of human HMGA2. The amino acid sequence is unavailable.
Positive control
SW579 cells. Colon carcinoma.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Post embryonic development >> Aging
Cancer >> Cell cycle >> Cell differentiation
Stem Cells >> Neural Stem Cells >> Intracellular
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Differentiation
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Metabolism
Epigenetics and Nuclear Signaling >> Histones >> HMGs
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
Applications
Show applications key
Our Abpromise guarantee covers the use of ab41878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
IP: Use at an assay dependent concentration.
WB: Use a concentration of 2 - 4 µg/ml.Detects a band of approximately 17 kDa (predicted molecular weight: 12 kDa).
Target
Function
Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2.
Involvement in disease
Note=A chromosomal aberration involving HMGA2 is associated with a subclass of benign mesenchymal tumors known as lipomas. Translocation t(3;12)(q27-q28;q13-q15) with LPP is shown in lipomas. HMGA2 is also fused with a number of other genes in lipomas.
Note=A chromosomal aberration involving HMGA2 is associated with pulmonary chondroid hamartomas. Translocation t(3;12)(q27-q28;q14-q15) with LPP is detected in pulmonary chondroid hamartomas.
Note=A chromosomal aberration involving HMGA2 is associated with parosteal lipomas. Translocation t(3;12)(q28;q14) with LPP is also shown in one parosteal lipoma.
Note=A chromosomal aberration involving HMGA2 is found in uterine leiomyoma. Translocation t(12;14)(q15;q23-24) with RAD51L1. Chromosomal rearrangements involving HMGA2 do not seem to be the principle pathobiological mechanism in uterine leiomyoma.
Sequence similarities
Belongs to the HMGA family.
Contains 3 A.T hook DNA-binding domains.
Developmental stage
Expressed predominantly during embryogenesis.
Post-translational
modifications
Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP52926
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HMGA 2 antibody
- BABL antibody
- High mobility group (nonhistone chromosomal) protein isoform I C antibody
see all
Database links
- Entrez Gene: 8091 Human
- Entrez Gene: 15364 Mouse
- Omim: 600698 Human
- SwissProt: P52926 Human
- SwissProt: P52927 Mouse
- Unigene: 505924 Human
- Unigene: 157190 Mouse
Anti-HMGA2 antibody images:
Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody (ab41878)
ICC/IF image of ab41878 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41878, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - HMGA2 antibody (ab41878)
Anti-HMGA2 antibody (ab41878) at 1 µg/ml + MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 12 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
Additional bands at : 42 kDa,74 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
HMGA2 contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Immunoprecipitation - Anti-HMGA2 antibody (ab41878)
HMGA2 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5ug of Rabbit polyclonal to HMGA2and 50µl of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10min under agitation. No antibody was added to the control (lane 2). HepG2 whole cell extractdiluted in RIPA buffer was added to each sample and incubated for 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab41878. Secondary: Clean blot (HRP conjugate) at 1/1000 dilution. Bands: 17kDa; HMGA2: 55kDa; Heavy Chains: Non Specific - 45 and 130kDa: We are unsure as to the identity of this extra band.
References for Anti-HMGA2 antibody (ab41878)
This product has been referenced in:
- Chen PSet al. miR-107 promotes tumor progression by targeting the let-7 microRNA in mice and humans. J Clin Invest 121:3442-55 (2011). WB; Mouse.Read more (PubMed: 21841313) »
- Watanabe Met al. HMGA-targeted phosphorothioate DNA aptamers increase sensitivity to gemcitabine chemotherapy in human pancreatic cancer cell lines. Cancer Lett : (2011). WB; Human.Read more (PubMed: 22036895) »
See all 3 publications for this product
Publishing research using ab41878? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"