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Products:Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
Overview
Product name
Anti-HMGA2 antibody
See all HMGA2 products (5) ...
Description
Rabbit polyclonal to HMGA2
Tested applications
ICC/IF, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to C-terminal of human HMGA2.
Positive control
SW579 cells. Breast carcinoma
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Purified IgA
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Post embryonic development >> Aging
Cancer >> Cell cycle >> Cell differentiation
Stem Cells >> Neural Stem Cells >> Intracellular
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Differentiation
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Metabolism
Epigenetics and Nuclear Signaling >> Histones >> HMGs
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 - 5 µg/ml.
IHC-P: 1/50
Target
Function
Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2.
Involvement in disease
Note=A chromosomal aberration involving HMGA2 is associated with a subclass of benign mesenchymal tumors known as lipomas. Translocation t(3;12)(q27-q28;q13-q15) with LPP is shown in lipomas. HMGA2 is also fused with a number of other genes in lipomas.
Note=A chromosomal aberration involving HMGA2 is associated with pulmonary chondroid hamartomas. Translocation t(3;12)(q27-q28;q14-q15) with LPP is detected in pulmonary chondroid hamartomas.
Note=A chromosomal aberration involving HMGA2 is associated with parosteal lipomas. Translocation t(3;12)(q28;q14) with LPP is also shown in one parosteal lipoma.
Note=A chromosomal aberration involving HMGA2 is found in uterine leiomyoma. Translocation t(12;14)(q15;q23-24) with RAD51L1. Chromosomal rearrangements involving HMGA2 do not seem to be the principle pathobiological mechanism in uterine leiomyoma.
Sequence similarities
Belongs to the HMGA family.
Contains 3 A.T hook DNA-binding domains.
Developmental stage
Expressed predominantly during embryogenesis.
Post-translational
modifications
Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP52926
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HMGA 2 antibody
- BABL antibody
- High mobility group (nonhistone chromosomal) protein isoform I C antibody
see all
Database links
- Entrez Gene: 8091 Human
- Omim: 600698 Human
- SwissProt: P52926 Human
- Unigene: 505924 Human
Anti-HMGA2 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HMGA2 antibody (ab52039)
Human breast carcinoma stained with Anti-HMGA2 antibody, ab52039 at 1:50 for 30 min at RT
Immunocytochemistry/ Immunofluorescence-HMGA2 antibody(ab52039)
ICC/IF image of ab52039 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52039, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-HMGA2 antibody (ab52039)
This product has been referenced in:
- Mu Get al. Correlation of overexpression of HMGA1 and HMGA2 with poor tumor differentiation, invasion, and proliferation associated with let-7 down-regulation in retinoblastomas. Hum Pathol 41:493-502 (2010). IHC-P; Human.Read more (PubMed: 20004941) »
See 1 publication for this product
Publishing research using ab52039? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"