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Anti-HMGB1 antibody [4C3] (ab11354)

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Overview

Product name

Anti-HMGB1 antibody [4C3]
See all HMGB1 products (14) ...

Description

Mouse monoclonal [4C3] to HMGB1

Tested applications

IHC-P, WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Hamster, Cow, Human, Pig

Immunogen

Full length native protein (purified) (Pig).

Positive control

HeLa Cell Lysate.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0% None
Constituents: 50% Glycerol, PBS

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Clone number

4C3

Isotype

IgG2a

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
Epigenetics and Nuclear Signaling >> Histones >> HMGs

Applications

Show applications key

Our Abpromise guarantee covers the use of ab11354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    IHC-P

     IHC-P: 1/50. Antigen ret...Read more →

    IHC-P: 1/50. Antigen retrieval is not necessary from customer feedback.

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    WB

    Five stars (2 Abreviews) WB: Use a concentration ...Read more →

    WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.Block with 5% milk for 45 min. Incubate antibody in PBS/Tween 20/0.25% milk. ab11354 was tested in IF using HeLa cells but gave widespread cytoplasmic as well as nuclear staining, and thus may not be suitable for this application.

Target

Function

DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Sequence similarities

Belongs to the HMGB family.
Contains 2 HMG box DNA-binding domains.

Cellular localization

Nucleus. Chromosome.

Target information above from: UniProt accessionP09429 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Amphoterin antibody
  • DKFZp686A04236 antibody
  • High mobility group 1 antibody
  • High Mobility Group Box 1 antibody
  • High mobility group protein 1 antibody
  • High mobility group protein B1 antibody
  • HMG-1 antibody
  • HMG1 antibody
  • HMG3 antibody
  • HMGB 1 antibody
  • HMGB1 antibody
  • Hmgb1 protein antibody
  • HMGB1_HUMAN antibody
  • Nonhistone chromosomal protein HMG1 antibody
  • SBP 1 antibody
  • SBP-1 antibody
  • Sulfoglucuronyl carbohydrate binding protein antibody
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Database links

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References for Anti-HMGB1 antibody [4C3] (ab11354)

This product has been referenced in:

  • Hornigold N  et al. Upregulation of Hic-5 in glomerulosclerosis and its regulation of mesangial cell apoptosis. Kidney Int 77:329-38 (2010). WB; Rat.Read more (PubMed: 20010548) »
  • Yang H  et al. Programmed necrosis induced by asbestos in human mesothelial cells causes high-mobility group box 1 protein release and resultant inflammation. Proc Natl Acad Sci U S A 107:12611-6 (2010). IHC-P; Mouse, Hamster.Read more (PubMed: 20616036) »

See all 7 publications for this product

Publishing research using ab11354? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"