Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Proteins and Peptides
- Secondary antibodies
- Lysates
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-HMGB1 antibody - Aminoterminal end
See all HMGB1 products (14) ...
Description
Rabbit polyclonal to HMGB1 - Aminoterminal end
Specificity
The immunogen shares 94 % homology with human HMGB2. It is therefore possible that HMGB2 will also be recongized by this antibody. In Western blotting, the HMGB2 band (24kD) will appear just below the HMBG1 band (24.8kD).
Tested applications
WB, IP, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Horse, Chicken, Cow, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Opossum
Immunogen
Synthetic peptide: GKGDPKKPRGKMSSYA conjugated to KLH, corresponding to N terminal amino acids 2-17 of Human HMGB1
GKGDPKKPRGKMSSYA
Positive control
3T3 cell lysates. HEK-293T cell lysates. PC12 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
Epigenetics and Nuclear Signaling >> Histones >> HMGs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab67281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 25 kDa.
IP: Use at an assay dependent dilution. Use 10µg.
ICC/IF: Use a concentration of 1 - 2 µg/ml. With paraformaldehyde-Triton® fixation.
Target
Function
DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.
Sequence similarities
Belongs to the HMGB family.
Contains 2 HMG box DNA-binding domains.
Cellular localization
Nucleus. Chromosome.
Target information above from: UniProt accessionP09429
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Amphoterin antibody
- DKFZp686A04236 antibody
- High mobility group 1 antibody
see all
Database links
- Entrez Gene: 282691 Cow
- Entrez Gene: 3146 Human
- Entrez Gene: 15289 Mouse
- Entrez Gene: 25459 Rat
- Omim: 163905 Human
- SwissProt: P36194 Chicken
- SwissProt: P10103 Cow
- SwissProt: Q08IE6 Horse
see all
References for Anti-HMGB1 antibody - Aminoterminal end (ab67281)
ab67281 has not yet been referenced specifically in any publications.
Publishing research using ab67281? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"