You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-HMGB1 antibody [EPR3507] (ab79823)

CodeSizePriceAbpointsAvailability
    
Add to basket
 

  • -

  •   
  •   
  •   

  •  

  •  
  •  

  •  

  •  
Updating...

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »

Overview

Product name

Anti-HMGB1 antibody [EPR3507]
See all HMGB1 products (14) ...

Description

Rabbit monoclonal [EPR3507] to HMGB1

Tested applications

WB, IP, Flow Cyt, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide corresponding to residues in human HMGB1.

Positive control

SKBR-3, HeLa and HepG2 cell lysates. Human kidney tissue.

General notes

Produced under U.S. Patent No. 5,675,063

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EPR3507

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
Epigenetics and Nuclear Signaling >> Histones >> HMGs

  • Western blot - HMGB1 antibody [EPR3507] (ab79823)Western blot - HMGB1 antibody [EPR3507] (ab79823) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HMGB1 antibody [EPR3507] (ab79823)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HMGB1 antibody [EPR3507] (ab79823) image (enlarge)

  • Flow Cytometry-HMGB1 antibody [EPR3507](ab79823)Flow Cytometry-HMGB1 antibody [EPR3507](ab79823) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab79823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Sequence similarities

Belongs to the HMGB family.
Contains 2 HMG box DNA-binding domains.

Cellular localization

Nucleus. Chromosome.

Target information above from: UniProt accessionP09429 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Amphoterin antibody
  • High mobility group 1 antibody
  • High mobility group box 1 antibody
  • High mobility group protein 1 antibody
  • High mobility group protein B1 antibody
  • HMG-1 antibody
  • HMG1 antibody
  • HMG3 antibody
  • HMGB 1 antibody
  • HMGB1 antibody
  • HMGB1_HUMAN antibody
  • SBP 1 antibody
  • Sulfoglucuronyl carbohydrate binding protein antibody
see all

Database links

see all

Anti-HMGB1 antibody [EPR3507] images:

  Western blot - HMGB1 antibody [EPR3507] (ab79823)

Western blot - HMGB1 antibody [EPR3507] (ab79823)

All lanes : Anti-HMGB1 antibody [EPR3507] (ab79823) at 1/50000 dilution

Lane 1 : SKBR-3 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
Goat anti-rabbit HRP conjugate at 1/2000 dilution

Predicted band size : 25 kDa
Observed band size : 25 kDa

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HMGB1 antibody [EPR3507] (ab79823)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HMGB1 antibody [EPR3507] (ab79823)

ab79823, at 1/250 dilution, staining HMGB1 in paraffin-embedded human kidney tissue by Immunohistochemistry.

  Flow Cytometry-HMGB1 antibody [EPR3507](ab79823)

Flow Cytometry-HMGB1 antibody [EPR3507](ab79823)

Overlay histogram showing HeLa cells stained with ab79823 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79823, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized with 0.1% PBS-Tween 20 used under the same conditions.

References for Anti-HMGB1 antibody [EPR3507] (ab79823)

This product has been referenced in:

  • Chen KB  et al. High-mobility Group Box-1 and Its Receptors Contribute to Proinflammatory Response in the Acute Phase of Spinal Cord Injury in Rats. Spine (Phila Pa 1976) : (2011). Rat.Read more (PubMed: 21343866) »

See 1 publication for this product

Publishing research using ab79823? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"