![Western blot - HMGB1 antibody [J2E1] (ab80246) image](#HMGB1-Primary-antibodies-ab80246-1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HMGB1 antibody [J2E1] (ab80246) image](#HMGB1-Primary-antibodies-ab80246-2.jpg)
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Product name
Anti-HMGB1 antibody [J2E1]
See all HMGB1 products (14) ...
Description
Mouse monoclonal [J2E1] to HMGB1
Tested applications
IHC-P, ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human) purified from High Five (Trichoplusia ni) insect cells
Positive control
Jurkat cell lysate HeLa cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
J2E1
Isotype
IgG2b
Light chain type
kappa
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
Epigenetics and Nuclear Signaling >> Histones >> HMGs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab80246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 - 1/300.
ELISA: Use at an assay dependent dilution.
WB: 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
Target
Function
DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.
Sequence similarities
Belongs to the HMGB family.
Contains 2 HMG box DNA-binding domains.
Cellular localization
Nucleus. Chromosome.
Target information above from: UniProt accessionP09429
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Amphoterin antibody
- High mobility group 1 antibody
- High mobility group box 1 antibody
see all
Database links
- Entrez Gene: 3146 Human
- Omim: 163905 Human
- SwissProt: P09429 Human
- Unigene: 434102 Human
- Unigene: 593339 Human
- Unigene: 596078 Human
Anti-HMGB1 antibody [J2E1] images:
Western blot - HMGB1 antibody [J2E1] (ab80246)
![Western blot - HMGB1 antibody [J2E1] (ab80246)](/ps/datasheet/images/80/ab80246/HMGB1-Primary-antibodies-ab80246-1.jpg)
All lanes : Anti-HMGB1 antibody [J2E1] (ab80246) at 1/500 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
Goat anti-mouse antibody conjugated to HRP
developed using the ECL technique
Predicted band size : 25 kDa
Observed band size : 25 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HMGB1 antibody [J2E1] (ab80246)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HMGB1 antibody [J2E1] (ab80246)](/ps/datasheet/images/80/ab80246/HMGB1-Primary-antibodies-ab80246-2.jpg)
ab80246 staining HMGB1 in human colon cancer tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in 0.1M sodium citrate buffer and incubated with primary antibody at 1/200 dilution, for 2 hours at 24°C. The tissue section was detected using DAB detection system.
References for Anti-HMGB1 antibody [J2E1] (ab80246)
ab80246 has not yet been referenced specifically in any publications.
Publishing research using ab80246? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"