Overview

  • Product nameAnti-HMGCS1 antibodySee all HMGCS1 primary antibodies ...
  • Description
    Mouse polyclonal to HMGCS1
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-520 of Human HMGCS1

  • Positive control
    • Human liver tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: 1/500 - 1/1000. Detects bands of approximately 57 and 65 kDa (predicted molecular weight: 57 kDa).
    Detection Antibody Pair.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThis enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.
    • PathwayMetabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.
    • Sequence similaritiesBelongs to the HMG-CoA synthase family.
    • Cellular localizationCytoplasm.
    • Target information above from: UniProt accession Q01581 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • 3 hydroxy 3 methylglutaryl Coenzyme A synthase 1 (soluble) antibody
      • 3-hydroxy-3-methylglutaryl coenzyme A synthase antibody
      • cytoplasmic antibody
      • EC 2.3.3.10 antibody
      • HMCS1_HUMAN antibody
      • HMG CoA synthase antibody
      • HMG-CoA synthase antibody
      • HMGCS antibody
      • HMGCS1 antibody
      • Hydroxymethylglutaryl CoA synthase, cytoplasmic antibody
      • Hydroxymethylglutaryl-CoA synthase antibody
      • MGC90332 antibody
      see all

    Anti-HMGCS1 antibody images

    • Anti-HMGCS1 antibody (ab67242) at 1/500 dilution + human liver tissue lysate at 25 µg/ml

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 57 kDa
      Observed band size : 57 kDa
    • All lanes : Anti-HMGCS1 antibody (ab67242) at 1/500 dilution

      Lane 1 : HMGCS1 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg/ml per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 57 kDa
      Observed band size : 65 kDa (why is the actual band size different from the predicted?)

    References for Anti-HMGCS1 antibody (ab67242)

    This product has been referenced in:
    • Martinez-Outschoorn UE  et al. Ketone bodies and two-compartment tumor metabolism: stromal ketone production fuels mitochondrial biogenesis in epithelial cancer cells. Cell Cycle 11:3956-63 (2012). Read more (PubMed: 23082721) »

    See 1 Publication for this product

    Product Wall

    Thank you for contacting us.
    We have antibodies to HMG CoA Synthase (HMGS) 3 Hydroxy 3 Methyl Glutaryl CoA Synthase only. The catalogue numbers are ab14302; http://www.abcam.com/HMGCS2-antibody-ab14302.html
    ab67242; http://www.abcam.com/HMGCS...

    Read More
    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (diffuse large B cell lymphoma cell line)
    Loading amount 1e+006 cells
    Specification diffuse large B cell lymphoma cell line
    Gel Running Conditions Reduced Denaturing (9%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    Submitted Nov 20 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"