Anti-HMGCS1 antibody (ab87246)

Overview

  • Product name
  • Description
    Rabbit polyclonal to HMGCS1
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 252-301 (DFTLNDFGFM IFHSPYCKLV QKSLARMLLN DFLNDQNRDK NSIYSGLEAF) of Human HMGCS1, NP_002121

  • Positive control
    • Transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab87246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

Target

Images

  • Anti-HMGCS1 antibody (ab87246) at 1 µg/ml + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 57 kDa
    Observed band size : 57 kDa
  • ICC/IF image of ab87246 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab87246, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab87246 staining HMGCS1 at 5 µg/ml in Human skeletal muscle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References

ab87246 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry these antibodies (anti-HLCS and anti-HMGCS1) did not perform as stated on the datashee...

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Thank you for contacting us.
We have antibodies to HMG CoA Synthase (HMGS) 3 Hydroxy 3 Methyl Glutaryl CoA Synthase only. The catalogue numbers are ab14302; http://www.abcam.com/HMGCS2-antibody-ab14302.html
ab67242; http://www.abcam.com/HMGCS...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (lymphoma cell line)
Loading amount
50 µg
Specification
lymphoma cell line
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Linfeng Chen

Verified customer

Submitted Mar 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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