HNF4 antibody [K9218] (ab41898)

Product Name 

HNF4 antibody [K9218]

Product type 

Primary antibodies

Description 

Mouse monoclonal [K9218] to HNF4

Immunogen 

Baculovirus-expressed recombinant fragment: MADYSAALDP AYTTLEFENV QVLTMGNDTS PSEGTNLNAP NSLGVSAL, corresponding to amino acids 3-49 of Human HNF4
BLAST 'MADYSAALDP AYTTLEFENV QVLTMGNDTS PSEGTNLNAP ' with or

Reacts with 

Hu, Ms, Rat

Predicted to react 
(species key)

Cow, Pig
(Due to sequence homology)

Specificity 

Recognizes human HNF4 alpha 1-6.

Tested applications 
(see key)

ELISA, GSA, ICC/IF, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: Use at a concentration of 0.1 µg/ml.
GSA: Use at an assay dependent dilution.
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: Use at a concentration of 10-20 µg/ml.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human liver hepatocytes and rat intestine epithelial cell.

Cellular localization 

Nuclear

Research areas 

Developmental Biology >> Organogenesis >> Gut development >> Liver development
Cardiovascular >> Atherosclerosis >> Diabetes associated
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Chromatin and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Other
Signal Transduction >> Metabolism >> Energy Metabolism

Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cytochromes
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Cardiovascular >> Blood >> Coagulation >> Extrinsic

Relevance 

The protein encoded by the HNF4 gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non insulin dependent diabetes mellitus type I. At least three different transcript variants encoding three different isoforms have been found for this gene.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

K9218

Isotype 

IgG2a

Storage buffer 

Physiological saline Preservative: 0.1% Sodium Azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from ascites via ammonium sulfate fractionation.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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HNF4 antibody [K9218] images:

References for HNF4 antibody [K9218] (ab41898)

  This product has been used in:

Haase A et al. Generation of induced pluripotent stem cells from human cord blood. Cell Stem Cell 5:434-41 (2009). ICC/IF; Human. PubMed: 19796623

If you publish research using ab41898 please let us know so that we can cite the reference on this datasheet.

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