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Western blot - HNF4 antibody (ab36175)
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Product Name
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HNF4 antibody
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See all HNF4 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to HNF4
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Immunogen
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Synthetic peptide: RLSKTLVDMDMADY (Human) N terminal.
BLAST 'RLSKTLVDMDMADY' with  or 
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Reacts with
(species key)
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Hu
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Specificity
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This antibody is expected to recognise the reported isoforms a, b and c (NP_849180.1; NP_000448.3; NP_849181.1 resp.).
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Tested applications
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/32000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 50 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Organogenesis >> Gut development >> Liver development
Cardiovascular >> Atherosclerosis >> Diabetes associated
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Chromatin and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Other
Signal Transduction >> Metabolism >> Energy Metabolism
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cytochromes
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Cardiovascular >> Blood >> Coagulation >> Extrinsic
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Relevance
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The protein encoded by the HNF4 gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non insulin dependent diabetes mellitus type I. At least three different transcript variants encoding three different isoforms have been found for this gene.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline. pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HNF4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for HNF4 antibody images, references, products related to ab36175 and other tools.
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HNF4 antibody images:
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 Western blot - HNF4 antibody (ab36175)
HNF4 antibody (ab36175) at 0.1 µg/ml + HepG2 lysate (35µg protein in RIPA buffer).
Predicted band size : 50 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
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Search PubMed (MEDLINE) for references to HNF4
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HNF4 antibody - more information
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Customer reviews (feedback) regarding HNF4 antibody |
Customer FAQs regarding HNF4 antibody |
Protocols for HNF4 antibody |
Price and availability of products related to HNF4 antibody |
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HNF4 antibody - related products:
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HNF4 antibody - other tools:
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Contact Abcam with a Technical Enquiry about HNF4 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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