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Products:Cardiovascular >> Blood >> Coagulation >> Extrinsic
Overview
Product name
Anti-HNF4 antibody
See all HNF4 products (8) ...
Description
Mouse monoclonal to HNF4
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: NDRQYDSRGR FGELLLLLPT LQSITWQMIE QIQFIKLFGM AKIDNLLQEM LLGGSPSDAP HAHHPLHPHL MQEHMGTNVI VANTMPTHLS NGQMCEWPRP , corresponding to amino acids 324-424 of Human HNF4
NDRQYDSRGR FGELLLLLPT LQSITWQMIE QIQFIKLFGM AKIDNLLQEM LLGGSPSDAP HAHHPLHPHL MQEHMGTNVI VANTMPTHLS NGQMCEWPRP
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Developmental Biology >> Organogenesis >> Gut development >> Liver development
Cardiovascular >> Atherosclerosis >> Diabetes associated
Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Other
Signal Transduction >> Metabolism >> Energy Metabolism
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cytochromes
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Cardiovascular >> Blood >> Coagulation >> Extrinsic
Applications
Show applications key
Our Abpromise guarantee covers the use of ab55223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 52 kDa.
ICC/IF: Use a concentration of 10 µg/ml
Target
Function
Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.
Involvement in disease
Defects in HNF4A are the cause of maturity-onset diabetes of the young type 1 (MODY1) [MIM:125850]; also symbolized MODY-1. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
Sequence similarities
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Post-translational
modifications
Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP41235
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ39654 antibody
- Hepatic nuclear factor 4 alpha antibody
- Hepatocyte nuclear factor 4 alpha antibody
see all
Database links
- Entrez Gene: 3172 Human
- Omim: 600281 Human
- SwissProt: P41235 Human
- Unigene: 116462 Human
Anti-HNF4 antibody images:
Western blot - HNF4 antibody (ab55223)
Predicted band size : 52 kDa
HNF4 antibody (ab55223) at 1ug/lane + HepG2 cell lysate at 25ug/lane.
Immunocytochemistry/ Immunofluorescence-HNF4 antibody(ab55223)
ab55223 at 10 ug/ml staining HNF4 in human cells by Immunocytochemistry/ Immunofluorescence.
References for Anti-HNF4 antibody (ab55223)
ab55223 has not yet been referenced specifically in any publications.
Publishing research using ab55223? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"