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All lanes : Anti-HNF4 (phospho S313) antibody (ab78356) at 1/500 dilution
Lane 1 : Extracts from HUVEC cells,
treated with EGF (200ng/ml, 30mins)
Lane 2 : Extracts from HUVEC cells,
treated with EGF (200ng/ml, 30mins) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 54 kDa
Observed band size : 54 kDa
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using ab78356 at a 1/50 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
Immunofluorescence analysis of LOVO cells using ab78356 at a 1/500 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
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