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Products:Cardiovascular >> Blood >> Coagulation >> Extrinsic
Overview
Product name
Anti-HNF4 (phospho S313) antibody
See all HNF4 products (8) ...
Description
Rabbit polyclonal to HNF4 (phospho S313)
Specificity
This antibody detects endogenous levels of HNF4 only when phosphorylated at serine 313.
Tested applications
WB, ELISA, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthesized phosphopeptide derived from human HNF4 around the phosphorylation site of serine 313 (L-R-SP-Q-V).
Positive control
Extracts from HUVEC cells. Human liver carcinoma tissue. LOVO cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Developmental Biology >> Organogenesis >> Gut development >> Liver development
Cardiovascular >> Atherosclerosis >> Diabetes associated
Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Other
Signal Transduction >> Metabolism >> Energy Metabolism
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cytochromes
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Cardiovascular >> Blood >> Coagulation >> Extrinsic
Applications
Show applications key
Our Abpromise guarantee covers the use of ab78356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
ELISA
ELISA: 1/20000ELISA: 1/20000
IHC-P: 1/50 - 1/100.
ICC/IF: 1/500 - 1/1000.
Target
Function
Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.
Involvement in disease
Defects in HNF4A are the cause of maturity-onset diabetes of the young type 1 (MODY1) [MIM:125850]; also symbolized MODY-1. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
Sequence similarities
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Post-translational
modifications
Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP41235
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ39654 antibody
- Hepatic nuclear factor 4 alpha antibody
- Hepatocyte nuclear factor 4 alpha antibody
see all
Database links
- Entrez Gene: 3172 Human
- Entrez Gene: 15378 Mouse
- Entrez Gene: 25735 Rat
- Omim: 600281 Human
- SwissProt: P41235 Human
- SwissProt: P49698 Mouse
- SwissProt: P22449 Rat
- Unigene: 116462 Human
see all
Anti-HNF4 (phospho S313) antibody images:
Western blot - HNF4 (phospho S313) antibody (ab78356)
All lanes : Anti-HNF4 (phospho S313) antibody (ab78356) at 1/500 dilution
Lane 1 : Extracts from HUVEC cells,
treated with EGF (200ng/ml, 30mins)
Lane 2 : Extracts from HUVEC cells,
treated with EGF (200ng/ml, 30mins) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 54 kDa
Observed band size : 54 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HNF4 (phospho S313) antibody (ab78356)
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using ab78356 at a 1/50 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
Immunocytochemistry/ Immunofluorescence - HNF4 (phospho S313) antibody (ab78356)
Immunofluorescence analysis of LOVO cells using ab78356 at a 1/500 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
References for Anti-HNF4 (phospho S313) antibody (ab78356)
ab78356 has not yet been referenced specifically in any publications.
Publishing research using ab78356? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"