Recombinant Anti-HOXB13 antibody [EPR17371] (ab201682)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17371] to HOXB13
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-HOXB13 antibody [EPR17371]
See all HOXB13 primary antibodies -
Description
Rabbit monoclonal [EPR17371] to HOXB13 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human prostate cancer lysate; LNCaP and PC-3 cell lysates. IHC-P: Human carcinoma of prostate tissue. IP: LNCaP cell lysate
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17371 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/5000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
|
|
IHC-P |
1/3000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
IP |
1/20.
|
Notes |
---|
WB
1/5000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). |
IHC-P
1/3000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/20. |
Target
-
Function
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. -
Sequence similarities
Belongs to the Abd-B homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 10481 Human
- Omim: 604607 Human
- SwissProt: Q92826 Human
- Unigene: 66731 Human
-
Alternative names
- Homeo box B13 antibody
- Homeobox B13 antibody
- Homeobox protein Hox B13 antibody
see all
Images
-
Immunohistochemical analysis of paraffin-embedded Human carcinoma of prostate tissue labeling HOXB13 with ab201682 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on Human carcinoma of prostate tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
All lanes : Anti-HOXB13 antibody [EPR17371] (ab201682) at 1/5000 dilution
Lane 1 : Human prostate cancer lysate
Lane 2 : Human fetal brain lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : Human fetal spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
HOXB13 is predominantly expressed in the prostate.
-
ab201682 (purified) at 1/20 dilution immunoprecipitating HOXB13 in LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10 µg.
Lane 1 (input): LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10 µg
Lane 2 (+): ab201682 & LnCaP whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab201682 in LnCaP whole cell lysate
For western blotting, ab201682 at 1/500 dilution (0.15 µg/mL) and veriBlot for IP secondary antibody (HRP) (ab131366) at 1/1000 dilution was used.
Blocking and diluting buffer: 5% NFDM /TBST. -
All lanes : Anti-HOXB13 antibody [EPR17371] (ab201682) at 1/5000 dilution
Lane 1 : LNCaP (Human prostate cancer cell line) cell lysate
Lane 2 : PC-3 (Human prostate cancer cell line) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
HOXB13 is predominantly expressed in the prostate.
-
Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling HOXB13 with ab201682 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Human transitional cell carcinoma of bladder represents a HOXB13 negative tissue. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling HOXB13 with ab201682 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Human cerebral cortex represents a HOXB13 negative tissue. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (5)
ab201682 has been referenced in 5 publications.
- Geng H et al. HOXB13 suppresses proliferation, migration and invasion, and promotes apoptosis of gastric cancer cells through transcriptional activation of VGLL4 to inhibit the involvement of TEAD4 in the Hippo signaling pathway. Mol Med Rep 24:N/A (2021). PubMed: 34396425
- Zhou S et al. Noncoding mutations target cis-regulatory elements of the FOXA1 plexus in prostate cancer. Nat Commun 11:441 (2020). PubMed: 31974375
- VanOpstall C et al. MEIS-mediated suppression of human prostate cancer growth and metastasis through HOXB13-dependent regulation of proteoglycans. Elife 9:N/A (2020). PubMed: 32553107
- Simons BW et al. A hemi-spleen injection model of liver metastasis for prostate cancer. Prostate 80:1263-1269 (2020). PubMed: 32761950
- Mazrooei P et al. Cistrome Partitioning Reveals Convergence of Somatic Mutations and Risk Variants on Master Transcription Regulators in Primary Prostate Tumors. Cancer Cell 36:674-689.e6 (2019). PubMed: 31735626