Overview
- Product nameAnti-HOXC5 antibodySee all HOXC5 primary antibodies ...
- DescriptionMouse monoclonal to HOXC5
- Tested applicationsELISA, WB more details
- Species reactivityReacts with: Recombinant Fragment
Predicted to work with: Mouse, Human - Immunogen
Recombinant fragment: MSSYVANSFY KQSPNIPAYN MQTCGNYGSA SEVQASRYCY GGLDLSITFP PPAPSNSLHG VDMAANPR, corresponding to amino acids 1-69 of Human HOXC5 with a 26kDa tag.
- Positive controlRecombinant fragment
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 1X PBS, pH 7.2 -
Concentration information loading... - PurityProtein A purified
- Clonality Monoclonal
- IsotypeIgG2a
- Light chain typekappa
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab76565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA: Use at an assay dependent dilution. Detection limit for recombinant HOXC5 is approximately 3ng/ml when used as a capture antibody. |
| WB | WB: Use at an assay dependent dilution. Detects a band of approximately 34 kDa (predicted molecular weight: 25 kDa). This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein. |
Target
- FunctionSequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
- Sequence similaritiesBelongs to the Antp homeobox family.
Contains 1 homeobox DNA-binding domain. - Cellular localizationNucleus.
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Database links
- Entrez Gene: 3222 Human
- Entrez Gene: 3222 Human
- Entrez Gene: 15424 Mouse
- Omim: 142973 Human
- SwissProt: Q00444 Human
- SwissProt: Q00444 Human
- SwissProt: P32043 Mouse
- Unigene: 549040 Human
- Unigene: 103825 Mouse
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Target information above from: UniProt accession
Q00444
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- CP11 antibodyhomeo box C5 antibodyhomeobox C5 antibody
- Homeobox protein CP11 antibodyhomeobox protein Hox C5 antibodyHomeobox protein Hox-3D antibodyHomeobox protein Hox-C5 antibodyHox 3D antibodyHOX3 antibodyHOX3D antibodyHOXC5 antibodyHXC5_HUMAN antibodyOTTHUMP00000217386 antibody
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Anti-HOXC5 antibody images
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![Western blot - HOXC5 antibody [leave blank] (ab76565)](http://a.abcam.com/ps/datasheet/images/76/ab76565/HOXC5-Primary-antibodies-ab76565-1.jpg)
Western blot - HOXC5 antibody [leave blank] (ab76565)Anti-HOXC5 antibody (ab76565) at 1/500 dilution + immunogen (recombinant fragment) at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 25 kDa
Observed band size : 34 kDa (why is the actual band size different from the predicted?)
References for Anti-HOXC5 antibody (ab76565)
ab76565 has not yet been referenced specifically in any publications.
