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ab4718 |
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Read our guarantee »Publishing research using ab9057? Please let us know so that we can cite the reference in this datasheet
ab9057 has been referenced in 8 publications.
Publishing research using ab9057? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-HP1 alpha antibody - Heterochromatin marker (ab9057) at 1/1000 dilution
Lane 1 : HeLa lysate
Lane 2 : HeLa lysate with
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L (HRP) at 1/2000 dilution
Predicted band size : 22 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
Detection of ab9057 in a blood sample. This result is compatible with other results obtained with other HP1 antibodies (Minc et al., 2000 Cytogenet Cell Genet 90:279 –284). (Manuela Silva, personal communication).
ab9057 staining HP1 alpha in human A549 cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.25% Triton and then blocked using 3% BSA for 1 hour at 18ºC. Samples were then incubated with primary antibody at 1/500 for 16 hours at 4ºC. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/800 dilution. Nuclei stained with DAPI (blue).
Image courtesy of an anonymous Abreview.
Anti-HP1 alpha antibody - Heterochromatin marker (ab9057) at 1/500 dilution +
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 90 seconds
4
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