HP1 alpha antibody - Heterochromatin marker (ab9057)

Product Name 

HP1 alpha antibody - Heterochromatin marker

Product type 

Primary antibodies

Description 

Rabbit polyclonal to HP1 alpha - Heterochromatin marker

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human HP1 alpha.

(Peptide available as ab4718.)

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ICC, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC: 1/500.
WB: 1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 22 kDa). Can be blocked with HP1 alpha peptide (ab4718).

In our hands we have been unsuccessful applying this antibody in ChIP.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: Hela lysate

Cellular localization 

Nuclear

Research areas 

Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Chromatin
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> Methyl Lysine

Relevance 

HP1 alpha is a component of heterochromatin. It recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic repression. HP1 alpha may interact with lamin B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane.
HP1 proteins are relatively small proteins (~25 kDa) with a conserved amino-terminal chromo domain and a structurally related carboxy-terminal motif, the chromo shadow domain. Both domains of HP1 are required
for binding to native chromatin in vivo, but they contribute differentially to binding in euchromatin and heterochromatin.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

This antibody was thought to work in plants, but the localisation patterns, though specific, were not co-localised with methylated Histone H3 K9 as is found in other species.

See below for HP1 alpha antibody - Heterochromatin marker images, references, products related to ab9057 and other tools.

HP1 alpha antibody - Heterochromatin marker images:

References for HP1 alpha antibody - Heterochromatin marker (ab9057)

  This product has been used in: (two most recent references)

Deng Z et al. TERRA RNA binding to TRF2 facilitates heterochromatin formation and ORC recruitment at telomeres. Mol Cell 35:403-13 (2009). WB; Human. PubMed: 19716786

Jeong S et al. Selective anchoring of DNA methyltransferases 3A and 3B to nucleosomes containing methylated DNA. Mol Cell Biol 29:5366-76 (2009). WB; Human. PubMed: 19620278

See all 4 publication references for this product.


If you publish research using ab9057 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding HP1 alpha antibody - Heterochromatin marker

Customer FAQs regarding HP1 alpha antibody - Heterochromatin marker

Protocols for HP1 alpha antibody - Heterochromatin marker

Price and availability of products related to HP1 alpha antibody - Heterochromatin marker

HP1 alpha antibody - Heterochromatin marker - related products: