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Overview
Product name
Anti-HP1 alpha antibody - Heterochromatin marker
See all HP1 alpha products (11) ...
Description
Rabbit polyclonal to HP1 alpha - Heterochromatin marker
Tested applications
ICC/IF, IHC-P, WB, ICCmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human HP1 alpha.
(Peptide available as ab4718.)
Positive control
WB: Hela lysate
General notes
This antibody was thought to work in plants, but the localisation patterns, though specific, were not co-localised with methylated Histone H3 K9 as is found in other species.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Chromatin
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> Methyl Lysine
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC
Application notes
ICC: 1/500.
WB: 1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 22 kDa). Can be blocked with HP1 alpha peptide (ab4718).
In our hands we have been unsuccessful applying this antibody in ChIP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Sequence similarities
Contains 2 chromo domains.
Post-translational
modifications
Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Ubiquitinated.
Cellular localization
Nucleus. Chromosome. Chromosome > centromere. Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase.
Target information above from: UniProt accessionP45973
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Antigen p25 antibody
- Cbx 5 antibody
- Cbx5 antibody
see all
Database links
- Entrez Gene: 23468 Human
- Omim: 604478 Human
- SwissProt: P45973 Human
- Unigene: 349283 Human
Anti-HP1 alpha antibody - Heterochromatin marker images:
All lanes : Anti-HP1 alpha antibody - Heterochromatin marker (ab9057) at 1/1000 dilution
Lane 1 : HeLa lysate
Lane 2 : HeLa lysate with HP1 alpha peptide (ab4718) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L (HRP) at 1/2000 dilution
Predicted band size : 22 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - HP1 alpha antibody - Heterochromatin marker (ab9057)
Detection of ab9057 in a blood sample. This result is compatible with other results obtained with other HP1 antibodies (Minc et al., 2000 Cytogenet Cell Genet 90:279 –284). (Manuela Silva, personal communication).
Immunocytochemistry/ Immunofluorescence - HP1 alpha antibody - Heterochromatin marker (ab9057)
ab9057 staining HP1 alpha in human A549 cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.25% Triton and then blocked using 3% BSA for 1 hour at 18°C. Samples were then incubated with primary antibody at 1/500 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/800 dilution. Nuclei stained with DAPI (blue).
Image courtesy of an anonymous Abreview.
References for Anti-HP1 alpha antibody - Heterochromatin marker (ab9057)
This product has been referenced in:
- Deutsch MJet al. The latent origin of replication of Epstein-Barr virus directs viral genomes to active regions of the nucleus. J Virol 84:2533-46 (2010). ICC/IF; Human.Read more (PubMed: 20032186) »
- Deng Zet al. TERRA RNA binding to TRF2 facilitates heterochromatin formation and ORC recruitment at telomeres. Mol Cell 35:403-13 (2009). WB; Human.Read more (PubMed: 19716786) »
See all 8 publications for this product
Publishing research using ab9057? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"