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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab16482? Please let us know so that we can cite the reference in this datasheet
ab16482 has been referenced in 1 publications.
- Luo SW et al. Regulation of heterochromatin remodelling and myogenin expression during muscle differentiation by FAK interaction with MBD2. EMBO J 28:2568-82 (2009). ICC/IF; Mouse. PubMed: 19661918
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - HP1 alpha antibody (ab16482)
Lane 1 - 6 : HP1 alpha antibody (ab16482) at 1/500
Lanes 4 - 6 : HP1 alpha peptide (ab4718) at 1 ug/ml
Lane 1 : HeLa Whole cell lysate
Lane 2 : HeLa Nuclear cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HeLa Whole cell lysate
Lane 5 : HeLa Nuclear cell lysate
Lane 6 : A431 cell lysate
Lysates at 20 ug/lane.
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Observed band size : 26kD
Immunocytochemistry/ Immunofluorescence - HP1 alpha antibody (ab16482)
ICC/IF image of ab16482 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab16482, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal donkey serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
HP1 alpha antibody for Immunocytochemistry/ Immunofluorescence in Human (16482)
1
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