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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Western blot - HP1 alpha antibody (ab16482)
Lane 1 - 6 : HP1 alpha antibody (ab16482) at 1/500
Lanes 4 - 6 : HP1 alpha peptide (ab4718) at 1 ug/ml
Lane 1 : HeLa Whole cell lysate
Lane 2 : HeLa Nuclear cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HeLa Whole cell lysate
Lane 5 : HeLa Nuclear cell lysate
Lane 6 : A431 cell lysate
Lysates at 20 ug/lane.
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Observed band size : 26kD
Immunocytochemistry/ Immunofluorescence - HP1 alpha antibody (ab16482)
ICC/IF image of ab16482 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab16482, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal donkey serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
HP1 alpha antibody for Immunocytochemistry/ Immunofluorescence in Human (16482)
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