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Overview
Product name
Anti-HP1 alpha antibody
See all HP1 alpha products (11) ...
Description
Goat polyclonal to HP1 alpha
Specificity
This antibody detects a strong band at 26kDa representing HP1 alpha.
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Chinese Hamster
Immunogen
Synthetic peptide, conjugated to KLH, correlating to aa from within regions 1-40 and 40-90 (separated with a GG linker) of Human HP1 alpha.
.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Chromatin
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> Methyl Lysine
Applications
Show applications key
Our Abpromise guarantee covers the use of ab16482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
ICC/IF: Use at an assay dependent concentration.
Previous customer feedback suggested that this antibody did not work in ICC/IF in interphase HeLa and SK-N-SH cells as no specific binding was detected(see Abreivews). More recent testing gave a positive result with this antibody. This difference may be due to different batches being tested. We welcome any further feedback about this antibody
WB: Use at a concentration of 2 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 22 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Sequence similarities
Contains 2 chromo domains.
Post-translational
modifications
Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Ubiquitinated.
Cellular localization
Nucleus. Chromosome. Chromosome > centromere. Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase.
Target information above from: UniProt accessionP45973
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Antigen p25 antibody
- Cbx 5 antibody
- Cbx5 antibody
see all
Database links
- Entrez Gene: 23468 Human
- Entrez Gene: 12419 Mouse
- Omim: 604478 Human
- SwissProt: P45973 Human
- SwissProt: Q61686 Mouse
- Unigene: 349283 Human
- Unigene: 262059 Mouse
Anti-HP1 alpha antibody images:
Western blot - HP1 alpha antibody (ab16482)
Lane 1 - 6 : HP1 alpha antibody (ab16482) at 1/500
Lanes 4 - 6 : HP1 alpha peptide (ab4718) at 1 ug/ml
Lane 1 : HeLa Whole cell lysate
Lane 2 : HeLa Nuclear cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HeLa Whole cell lysate
Lane 5 : HeLa Nuclear cell lysate
Lane 6 : A431 cell lysate
Lysates at 20 ug/lane.
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Observed band size : 26kD
Immunocytochemistry/ Immunofluorescence - HP1 alpha antibody (ab16482)
ICC/IF image of ab16482 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab16482, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal donkey serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-HP1 alpha antibody (ab16482)
This product has been referenced in:
- Luo SWet al. Regulation of heterochromatin remodelling and myogenin expression during muscle differentiation by FAK interaction with MBD2. EMBO J 28:2568-82 (2009). ICC/IF; Mouse.Read more (PubMed: 19661918) »
See 1 publication for this product
Publishing research using ab16482? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"