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Anti-HP1 alpha antibody [mAbcam 14298] - Heterochromatin marker (ab14298) at 5 µg/ml + HeLa whole cell lysate at 20 µg
Secondary
Goat anti-Mouse IgG HRP conjugate at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 22 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutes
Blocked with 3% milk.
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