HP1 alpha antibody [mAbcam 14298] - Heterochromatin marker (ab14298)

Product Name 

HP1 alpha antibody [mAbcam 14298] - Heterochromatin marker

Product type 

Primary antibodies

Description 

Mouse monoclonal [mAbcam 14298] to HP1 alpha - Heterochromatin marker

Immunogen 

Synthetic peptide, conjugated to KLH, correlating to aa from within regions 1-40 and 40-90 (separated with a GG linker) of Human HP1 alpha.

.(Peptide available as ab4718.)

Reacts with 

Hu

Predicted to react 
(species key)

Ms
(Due to sequence homology)

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 5 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 22 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Chromatin
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> Methyl Lysine

Relevance 

HP1 alpha is a component of heterochromatin. It recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic repression. HP1 alpha may interact with lamin B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane.
HP1 proteins are relatively small proteins (~25 kDa) with a conserved amino-terminal chromo domain and a structurally related carboxy-terminal motif, the chromo shadow domain. Both domains of HP1 are required
for binding to native chromatin in vivo, but they contribute differentially to binding in euchromatin and heterochromatin.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

mAbcam 14298

Myeloma 

Sp2/0-Ag14

Isotype 

IgG1

Purity 

Protein A purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: Tris HCl, 500mM Sodium chloride, 200mM Sodium citrate. pH 7.5

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.900 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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