![Western blot - HP1 alpha antibody [mAbcam 14298] (ab14298) image](#ab14298_1.jpg)
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Overview
Product name
Anti-HP1 alpha antibody [mAbcam 14298] - Heterochromatin marker
See all HP1 alpha products (11) ...
Description
Mouse monoclonal [mAbcam 14298] to HP1 alpha - Heterochromatin marker
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide, conjugated to KLH, correlating to aa from within regions 1-40 and 40-90 (separated with a GG linker) of Human HP1 alpha.
. (Peptide available as ab4718.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: Tris HCl, 500mM Sodium chloride, 200mM Sodium citrate. pH 7.5
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
mAbcam 14298
Myeloma
Sp2/0-Ag14
Isotype
IgG1
Light chain type
unknown
Research areas
Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Chromatin
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> Methyl Lysine
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Western blot - HP1 alpha antibody [mAbcam 14298] (ab14298)
![Western blot - HP1 alpha antibody [mAbcam 14298] (ab14298) image](/ps/datasheet/Images/14/ab14298/ab14298_1.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab14298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 5 µg/mlDetects a band of approximately 18 kDa (predicted molecular weight: 22 kDa).
Target
Function
Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Sequence similarities
Contains 2 chromo domains.
Post-translational
modifications
Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Ubiquitinated.
Cellular localization
Nucleus. Chromosome. Chromosome > centromere. Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase.
Target information above from: UniProt accessionP45973
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Antigen p25 antibody
- Antigen p25 antibody
- Cbx 5 antibody
see all
Database links
- Entrez Gene: 23468 Human
- Entrez Gene: 12419 Mouse
- Omim: 604478 Human
- SwissProt: P45973 Human
- SwissProt: Q61686 Mouse
- Unigene: 349283 Human
- Unigene: 262059 Mouse
Anti-HP1 alpha antibody [mAbcam 14298] - Heterochromatin marker images:
Western blot - HP1 alpha antibody [mAbcam 14298] (ab14298)
Anti-HP1 alpha antibody [mAbcam 14298] - Heterochromatin marker (ab14298) at 5 µg/ml + HeLa whole cell lysate at 20 µg
Secondary
Goat anti-Mouse IgG HRP conjugate at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 22 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutes
Blocked with 3% milk.
References for Anti-HP1 alpha antibody [mAbcam 14298] - Heterochromatin marker (ab14298)
ab14298 has not yet been referenced specifically in any publications.
Publishing research using ab14298? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"