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All lanes : Anti-HP1 alpha antibody - Heterochromatin marker (ab9057) at 1/1000 dilution
Lane 1 : HeLa lysate
Lane 2 : HeLa lysate with
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L (HRP) at 1/2000 dilution
Predicted band size : 22 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
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