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Overview
Product name
HP1 alpha peptide
See all HP1 alpha products (3) ...
Protein description
Synthetic peptide, , correlating to aa from within regions 1-40 and 40-90 (separated with a GG linker) of Human HP1 alpha. (Note: the amino acid sequence is proprietary). This peptide was used as an immunogen for ab9057 - HP1 alpha antibody - Heterochromatin marker and ab14298 - HP1 alpha antibody [mAbcam 14298].
Protein length
191 amino acids
Expression host
CHO
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Information available upon request.
Concentration
Concentration information loading...
Research areas
Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Chromatin
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> Methyl Lysine
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking
Application notes
BL: Use at a concentration of 1 µg/ml.
This peptide can be used with studies using ab16482, ab9057 and ab14298.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Sequence similarities
Contains 2 chromo domains.
Post-translational
modifications
Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Ubiquitinated.
Cellular localization
Nucleus. Chromosome. Chromosome > centromere. Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase.
Target information above from: UniProt accessionP45973
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
HP1 alpha peptide images:
All lanes : Anti-HP1 alpha antibody - Heterochromatin marker (ab9057) at 1/1000 dilution
Lane 1 : HeLa lysate
Lane 2 : HeLa lysate with HP1 alpha peptide (ab4718) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L (HRP) at 1/2000 dilution
Predicted band size : 22 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
References for HP1 alpha peptide (ab4718)
ab4718 has not yet been referenced specifically in any publications.
Publishing research using ab4718? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"