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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab45270? Please let us know so that we can cite the reference in this datasheet
ab45270 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - HP1 gamma (phospho S83) antibody (ab45270)
All lanes : Anti-HP1 gamma (phospho S83) antibody (ab45270) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 8 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with
Lane 9 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with
Lane 10 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with
Lane 11 : HEK293 Human embryonic kidney cell line Whole Cell Lysate with
Lane 12 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with
Lane 13 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate (ab28419) with
Lane 14 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate (ab45968) with
Lane 15 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with
Lane 16 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with
Lane 17 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with
Lane 18 : HEK293 Human embryonic kidney cell line Whole Cell Lysate with
Lane 19 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with
Lane 20 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate with
Lane 21 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate with
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 21 kDa
Observed band size : 24 kDa (why is the actual band size different from the predicted?)
Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - HP1 gamma (phospho S83) antibody (ab45270)
ICC/IF image of ab45270 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab45270, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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