Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Overview
Product name
Anti-HP1 gamma (phospho S83) antibody
See all HP1 gamma products (11) ...
Description
Rabbit polyclonal to HP1 gamma (phospho S83)
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Cow, Orangutan
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human HP1 gamma, phosphorylated at S83.
(Peptide available as ab45386.)
Positive control
ab45270 gave a positive result in the following Human Whole Cell Lysates: Hela, Jurkat, A431, HEK293, HepG2, MCF-7 and SHSY-5Y.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab45270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 24 kDa (predicted molecular weight: 21 kDa).
Target
Function
Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Sequence similarities
Contains 2 chromo domains.
Post-translational
modifications
Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Cellular localization
Nucleus. Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis.
Target information above from: UniProt accessionQ13185
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CBX 3 antibody
- CBX3 antibody
- CBX3_HUMAN antibody
see all
Database links
- Entrez Gene: 11335 Human
- Entrez Gene: 12417 Mouse
- Omim: 604477 Human
- SwissProt: Q13185 Human
- SwissProt: P23198 Mouse
- SwissProt: Q5R6X7 Orangutan
- Unigene: 381189 Human
- Unigene: 280968 Mouse
see all
Anti-HP1 gamma (phospho S83) antibody images:
Western blot - HP1 gamma (phospho S83) antibody (ab45270)
All lanes : Anti-HP1 gamma (phospho S83) antibody (ab45270) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 8 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with HP1 gamma peptide (ab51840) at 1 µg/ml
Lane 9 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with HP1 gamma peptide (ab51840) at 1 µg/ml
Lane 10 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with HP1 gamma peptide (ab51840) at 1 µg/ml
Lane 11 : HEK293 Human embryonic kidney cell line Whole Cell Lysate with HP1 gamma peptide (ab51840) at 1 µg/ml
Lane 12 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with HP1 gamma peptide (ab51840) at 1 µg/ml
Lane 13 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate (ab28419) with HP1 gamma peptide (ab51840) at 1 µg/ml
Lane 14 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate (ab45968) with HP1 gamma peptide (ab51840) at 1 µg/ml
Lane 15 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with HP1 gamma peptide - phospho S83 (ab45386) at 1 µg/ml
Lane 16 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with HP1 gamma peptide - phospho S83 (ab45386) at 1 µg/ml
Lane 17 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with HP1 gamma peptide - phospho S83 (ab45386) at 1 µg/ml
Lane 18 : HEK293 Human embryonic kidney cell line Whole Cell Lysate with HP1 gamma peptide - phospho S83 (ab45386) at 1 µg/ml
Lane 19 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with HP1 gamma peptide - phospho S83 (ab45386) at 1 µg/ml
Lane 20 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate with HP1 gamma peptide - phospho S83 (ab45386) at 1 µg/ml
Lane 21 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate with HP1 gamma peptide - phospho S83 (ab45386) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 21 kDa
Observed band size : 24 kDa (why is the actual band size different from the predicted?)
Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - HP1 gamma (phospho S83) antibody (ab45270)
ICC/IF image of ab45270 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab45270, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-HP1 gamma (phospho S83) antibody (ab45270)
ab45270 has not yet been referenced specifically in any publications.
Publishing research using ab45270? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"