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Western blot - HP1 gamma antibody (ab79304)
(enlarge)
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Product Name
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HP1 gamma (phospho S93) antibody
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See all HP1 gamma antibodies (10)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to HP1 gamma (phospho S93)
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Immunogen
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Synthesized non-phosphopeptide derived from human HP1 gamma around the phosphorylation site of serine 93 (RKSPLS).
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat
(Due to sequence homology)
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Specificity
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Detects endogenous levels of total HP1 gamma protein.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/40000.
WB: 1/500 - 1/1000. Detects a band of approximately 21 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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293 nuclear lysate
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> Methyl Lysine
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> Methylated DNA
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Relevance
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HP1 gamma appears to be involved in transcriptional silencing in heterochromatin-like complexes. It recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. HP1 gamma may contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR) and involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Ca2+ and Mg2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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Purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HP1 gamma (phospho S93) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for HP1 gamma (phospho S93) antibody images, references, products related to ab79304 and other tools.
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HP1 gamma (phospho S93) antibody images:
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 Western blot - HP1 gamma antibody (ab79304)
All lanes : HP1 gamma (phospho S93) antibody (ab79304) at 1/500 dilution
Lane 1 : Extracts from 293 cells with no immunizing peptide
Lane 2 : Extracts from 293 cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Observed band size : 21 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to HP1 gamma
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HP1 gamma (phospho S93) antibody - more information
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Customer reviews (feedback) regarding HP1 gamma (phospho S93) antibody |
Customer FAQs regarding HP1 gamma (phospho S93) antibody |
Protocols for HP1 gamma (phospho S93) antibody |
Price and availability of products related to HP1 gamma (phospho S93) antibody |
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HP1 gamma (phospho S93) antibody - related products:
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HP1 gamma (phospho S93) antibody - other tools:
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Contact Abcam with a Technical Enquiry about HP1 gamma (phospho S93) antibody |
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