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Overview

  • Product nameHP1 gamma protein (His tag)See all HP1 gamma proteins and peptides ...
  • Protein descriptionRecombinant full-length Human HP1 gamma (1-183aa) with N-terminal His-tag (NP_057671, Q13185). 207 aa, 23.4 kDa inclusive of tag.
  • Uniprot accessionQ13185
  • Molecular weight23.400kDa inclusive of tags
  • Protein length183 amino acids
  • Expression hostE. coli

    • Properties

  • Purity> 90 % by SDS-PAGE
  • Purification notesPurified by using conventional chromatography.
  • Mass spectrometryMALDI-TOF
  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 8.00
    Constituents: 0.02% DTT, 0.32% Tris HCl, 20% Glycerol, 0.58% Sodium chloride
  • Concentration information loading...
  • Sequence notesMGSSHHHHHH SSGLVPRGSH MGSHMASNKT TLQKMGKKQN GKSKKVEEAE PEEFVVEKVL DRRVVNGKVE YFLKWKGFTD ADNTWEPEEN LDCPELIEAF LNSQKAGKEK DGTKRKSLSD SESDDSKSKK KRDAADKPRG FARGLDPERI IGATDSSGEL MFLMKWKDSD EADLVLAKEA NMKCPQIVIA FYEERLTWHS CPEDEAQ
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab124548 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent concentration.
    MS MS: Use at an assay dependent concentration.
  • Application notesThis peptide can be used with studies using ab10480,ab40827,ab115221 and ab154195.

    • Protein info

    • Alternative names
        CBX 3CBX3CBX3_HUMAN
        Chromobox homolog 3Chromobox homolog 3 (HP1 gamma homolog, Drosophila)Chromobox protein homolog 3GAMMAHECHHeterochromatin like protein 1Heterochromatin protein 1 homolog gammaHeterochromatin protein HP1 gammaHP1 gammaHP1 gamma homologHP1Hs gammaModifier 2 protein
      see all
  • FunctionSeems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
  • Sequence similaritiesContains 2 chromo domains.
  • Post-translational
    modifications    Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
  • Cellular localizationNucleus. Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis.

    • Target information above from: UniProt accession Q13185 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    HP1 gamma protein (His tag) images

    • SDS-PAGE - HP1 gamma protein (ab124548)
      SDS-PAGE - HP1 gamma protein (ab124548)
      15% SDS-PAGE (3 µg loaded)

    References for HP1 gamma protein (His tag) (ab124548)

    ab124548 has not yet been referenced specifically in any publications.

    Publishing research using ab124548 ? Please let us know so that we can cite the reference in this datasheet

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