Anti-HP13 antibody (ab21808)

Product name

Anti-HP13 antibody

Description

Mouse polyclonal to HP13

Tested applications

WBmore details

Cross reactivity

reacts with Plasmodium falciparum. Not yet tested in other species.

Immunogen

Fusion protein: YLIFEEIKDDNKKDHDDERSLRKDHNNDDERSLRKDHNND DEKGDKKDHDNDDEKGNRKDNNNEEEKGDKKDHNNDDDKK GNIKDNNNEEEKKTNDDNKH, corresponding to amino acids 251/350 of HP13 from Plasmodium falciparum.

YLIFEEIKDD NKKDHDDERS LRKDHNNDDE RSLRKDHNND DEKGDKKDHD NDDEKGNRKD NNNEEEKGDK KDHNNDDDKK GNIKDNNNEE EKKTNDDNKH

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Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Constituents: 50% Glycerol

Purity

Whole antiserum

Primary antibody notes

This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al.PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Clonality

Polyclonal

Isotype

IgG

Research areas

Microbiology >> Organism >> Eukaryotic Microorganism >> Protozoa >> Plasmodium