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Products:Signal Transduction >> Protein Trafficking >> Organelle Proteins
Overview
Product name
Anti-HPS4 antibody
Description
Mouse polyclonal to HPS4
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Fusion protein: GHFAFLHVPVPDGRAPYCKASLSASSSLEPTPPEDTAISS LRPPSA, corresponding to amino acids 385/430 of Human HPS4
GHFAFLHVPV PDGRAPYCKA SLSASSSLEP TPPEDTAISS LRPPSA
General notes
Produced from outbred CD1 mice
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Constituents: 50% Glycerol
Purity
Whole antiserum
Primary antibody notes
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al.PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Organelle Proteins
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Western blot - HPS4 antibody (ab22147)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab22147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000. Predicted molecular weight: 77 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Target
Relevance
Hermansky-Pudlak syndrome is a disorder of organelle biogenesis in which oculocutaneous albinism, bleeding, and pulmonary fibrosis result from defects of melanosomes, platelet dense granules, and lysosomes. Mutations in HPS4 gene as well as several others can cause this syndrome. HPS4 appears to be important in organelle biogenesis and is similar to the mouse 'light ear' protein. Five transcript variants encoding different isoforms have been found for this gene. In addition, transcript variants utilizing alternative polyadenylation signals exist.
Cellular localization
lysosome, melanosome, membrane fraction and platelet dense granule
Alternative names
- Hermansky Pudlak syndrome 4 protein antibody
- Light ear protein homolog antibody
Database links
- Entrez Gene: 89781 Human
- Omim: 606682 Human
- SwissProt: Q6P1K3 Human
- SwissProt: Q9NQG7 Human
- Unigene: 474436 Human
Anti-HPS4 antibody images:
Western blot - HPS4 antibody (ab22147)
All lanes : Anti-HPS4 antibody (ab22147) at 1/1000 dilution
Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a
negative control fusion protein with an irrelevant antigen at 20 ug
Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the
antigen fusion protein at 20 ug
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at
1/5000 dilution
Predicted band size : 77 kDa
The molecular weight of the band on the western blot does not correspond to the predicted band size above (predicted from the molecular weight of the natural protein) because of the additional mass of the fusion and because the fusion protein only contains a partial fragment of the gene.
References for Anti-HPS4 antibody (ab22147)
ab22147 has not yet been referenced specifically in any publications.
Publishing research using ab22147? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"