Anti-HS6ST2 antibody (ab122220)
Key features and details
- Rabbit polyclonal to HS6ST2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-HS6ST2 antibody -
Description
Rabbit polyclonal to HS6ST2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: RKRQEQRKFL KGRLLQTHFQ SQGQGQSQNP NQNQSQNPNP NANQNLTQNL MQNLTQSLSQ KENRESPKQN SGKEQNDNTS NGTND, corresponding to amino acids 512-596 of Human HS6ST2.
-
Positive control
- Human cerebral cortex tissue; RT-4 and U-251 MG lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
1/250 - 1/500.
|
Notes |
---|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/250 - 1/500. |
Target
-
Function
6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate. -
Sequence similarities
Belongs to the sulfotransferase 6 family. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 90161 Human
- Omim: 300545 Human
- SwissProt: Q96MM7 Human
- Unigene: 385956 Human
-
Alternative names
- H6ST2_HUMAN antibody
- Heparan-sulfate 6-O-sulfotransferase 2 antibody
- HS6ST-2 antibody
see all
Images
-
All lanes : Anti-HS6ST2 antibody (ab122220) at 1/250 dilution
Lane 1 : RT-4 lysate
Lane 2 : U-251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique. -
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122220 1-4 µg/ml. Cells treated with PFA/Triton X-100.
-
ab122220, at 1/125 dilution, staining HS6ST2 in paraffin-embedded Human Cerebral cortex tissue by Immunohistochemistry.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab122220 has been referenced in 3 publications.
- Zhang Y et al. Role of lncRNA FAM83H antisense RNA1 (FAM83H-AS1) in the progression of non-small cell lung cancer by regulating the miR-545-3p/heparan sulfate 6-O-sulfotransferase (HS6ST2) axis. Bioengineered 13:6476-6489 (2022). PubMed: 35260044
- Guo Y et al. Downregulation of HS6ST2 by miR-23b-3p enhances matrix degradation through p38 MAPK pathway in osteoarthritis. Cell Death Dis 9:699 (2018). PubMed: 29899528
- Jin Y et al. Overexpression of HS6ST2 is associated with poor prognosis in patients with gastric cancer. Oncol Lett 14:6191-6197 (2017). PubMed: 29113266