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Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab84273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).

Target

  • FunctionCatalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol.
  • Tissue specificityWidely expressed. Highest expression in liver.
  • Involvement in diseaseDefects in HSD11B1 are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS).
  • Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
  • Post-translational
    modifications    Glycosylated.
  • Cellular localizationEndoplasmic reticulum membrane.

    • Target information above from: UniProt accession P28845 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        11 beta HSD 1 antibody11 beta HSD1 antibody11 beta hydroxysteroid dehydrogenase 1 antibody
        11 DH antibody11-beta hydroxysteroid dehydrogenase, type 1 antibody11-beta-HSD1 antibody11-beta-hydroxysteroid dehydrogenase 1 antibody11-DH antibody11DH antibodyCorticosteroid 11 beta dehydrogenase isozyme 1 antibodyCorticosteroid 11-beta-dehydrogenase isozyme 1 antibodyCORTRD2 antibodyDHI1_HUMAN antibodyHDL antibodyHSD 11 antibodyHSD11 antibodyHSD11B antibodyHSD11B1 antibodyHSD11L antibodyHydroxysteroid (11 beta) dehydrogenase antibodyHydroxysteroid (11 beta) dehydrogenase 1 antibodyMGC13539 antibodySDR26C1 antibodyshort chain dehydrogenase/reductase family 26C, member 1 antibody
      see all

    Anti-HSD11B1 antibody images

    • Western blot - HSD11B1 antibody (ab84273)
      Western blot - HSD11B1 antibody (ab84273)
      Anti-HSD11B1 antibody (ab84273) at 1 µg/ml + Liver (Human) Tissue Lysate - adult normal tissue (ab29889) at 10 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 32 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 47 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 30 seconds

    References for Anti-HSD11B1 antibody (ab84273)

    ab84273 has not yet been referenced specifically in any publications.

    Publishing research using ab84273 ? Please let us know so that we can cite the reference in this datasheet

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