Overview

  • Product nameAnti-HSD3B1 antibodySee all HSD3B1 primary antibodies ...
  • Description
    Rabbit polyclonal to HSD3B1
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cynomolgus Monkey, Rhesus monkey
  • Immunogen

    Synthetic peptide: MTGWSCLVTG AGGFLGQRII RLLVKEKELK EIRVLDKAFG PELREEFSKL, corresponding to N terminal amino acids 1-50 of Human HSD3B1 (NP_000853).

  • Positive control
    • Human placenta lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

Target

  • Function3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone.
  • Tissue specificityPlacenta and skin. Predominantly expressed in mammary gland tissue.
  • PathwayLipid metabolism; steroid biosynthesis.
  • Sequence similaritiesBelongs to the 3-beta-HSD family.
  • Cellular localizationEndoplasmic reticulum membrane. Mitochondrion membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 beta and steroid delta isomerase 1 antibody
    • 3 beta and steroid hydroxy delta 5 steroid dehydrogenase antibody
    • 3 beta HSD I antibody
    • 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I antibody
    • 3-beta-HSD I antibody
    • 3-beta-hydroxy-5-ene steroid dehydrogenase antibody
    • 3BETAHSD antibody
    • 3BH antibody
    • 3BHS1_HUMAN antibody
    • 3BHSD antibody
    • Delta-5-3-ketosteroid isomerase antibody
    • HGNC:5217 antibody
    • HSD3B antibody
    • HSD3B1 antibody
    • HSDB3 antibody
    • HSDB3A antibody
    • Hydroxy delta 5 steroid dehydrogenase 3 beta and steroid delta isomerase 1 antibody
    • Hydroxy delta 5 steroid dehydrogenase antibody
    • Progesterone reductase antibody
    • SDR11E1 antibody
    • Steroid Delta-isomerase antibody
    • trophoblast antigen antibody
    • Trophoblast antigen FDO161G antibody
    see all

Anti-HSD3B1 antibody images

  • Anti-HSD3B1 antibody (ab80363) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa
    Observed band size : 42 kDa

References for Anti-HSD3B1 antibody (ab80363)

This product has been referenced in:
  • Li X  et al. CCAAT/enhancer-binding protein ß is involved in the breed-dependent transcriptional regulation of 3ß-hydroxysteroid dehydrogenase/?(5)-?(4)-isomerase in adrenal gland of preweaning piglets. J Steroid Biochem Mol Biol 138C:273-280 (2013). Read more (PubMed: 23831357) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rhesus monkey Tissue sections (Testis)
Specification Testis
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

Submitted Aug 19 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"