![Immunoprecipitation - Anti-HSDL2 antibody [2A11BG6AH6 ] (ab110298) image](#HSDL2-Primary-antibodies-ab110298-5.gif)
![Immunocytochemistry - Anti-HSDL2 antibody [2A11BG6AH6 ] (ab110298) image](#HSDL2-Primary-antibodies-ab110298-4.gif)
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Product name
Anti-HSDL2 antibody [2A11BG6AH6 ]
See all HSDL2 products (3) ...
Description
Mouse monoclonal [2A11BG6AH6 ] to HSDL2
Tested applications
ICC, IP, In-Cell ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Human liver mitochondria.
Positive control
Hela cell and human liver mitochondria.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
Purity near homogeneity as judge by SDS-PAGE. The antibody was produced in-vitro using hybridomas grown in serum-free medium and then purified by biochemical fractionation
Clonality
Monoclonal
Clone number
2A11BG6AH6
Isotype
IgG1
Light chain type
kappa
Research areas
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Peroxisome
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: Use a concentration of 10 µg/ml.
IP: Use at an assay dependent dilution.
In-Cell ELISA: Use a concentration of 0.4 µg/ml. (0.4 µg/well)
Target
Function
Has apparently no steroid dehydrogenase activity.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Contains 1 SCP2 domain.
Cellular localization
Peroxisome.
Target information above from: UniProt accessionQ6YN16
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- C9orf99 antibody
- FLJ25855 antibody
- hsdl2 antibody
see all
Database links
- Entrez Gene: 84263 Human
- SwissProt: Q6YN16 Human
- Unigene: 59486 Human
Anti-HSDL2 antibody [2A11BG6AH6 ] images:
Immunoprecipitation - Anti-HSDL2 antibody [2A11BG6AH6 ] (ab110298)
![Immunoprecipitation - Anti-HSDL2 antibody [2A11BG6AH6 ] (ab110298)](/ps/datasheet/images/110/ab110298/HSDL2-Primary-antibodies-ab110298-5.gif)
As an IP antibody, HSDL2 antibody [2A11BG6AH6 ] (ab110298) works with human sample only.
Immunocytochemistry - Anti-HSDL2 antibody [2A11BG6AH6 ] (ab110298)
![Immunocytochemistry - Anti-HSDL2 antibody [2A11BG6AH6 ] (ab110298)](/ps/datasheet/images/110/ab110298/HSDL2-Primary-antibodies-ab110298-4.gif)
Immunocytochemistry image of HSDL2 stained human Hela cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with HSDL2 antibody [2A11BG6AH6 ](ab110298) at 10 µg/ml for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps.
References for Anti-HSDL2 antibody [2A11BG6AH6 ] (ab110298)
ab110298 has not yet been referenced specifically in any publications.
Publishing research using ab110298? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"