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Product name
HSP60 Human ELISA Kit
Specificity
Species– Human reactive. Non-reactive with mouse and rat. Others untested.
Precision
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| Overall | 4 | 2.1% |
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| Overall | 3 | 6.7% |
Tests
1 x 96 well plate
Sample type
Tissue Extracts, Cell Lysate
Assay type
Sandwich
Cross reactivity
Reacts with
Human
Product overview
ab124534 HSP60 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of HSP60 protein a 60 kDa protein encoded by the Human HSPD1 gene, in cell and tissue lysates. The assay employs an HSP60 specific antibody coated onto well plate strips.
Standards and samples are pipetted into the wells and analyte present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti-HSP60 primary detector antibody is added. After washing away unbound primary detector antibody, HRP-conjugated secondary detector antibody specific for the primary detector antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of analyte bound. The developing blue color is measured at 600 nm.
Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
Notes
Typical working ranges:
HepG2 cell extract: 0.39 - 200 µg/mL
Hela cell extract: 3.125 - 200 µg/mL
Store all components at 4°C. This kit is stable for at least 6 months from receipt. Unused microplate strips should be returned to the pouch containing the desiccant and resealed.
HepG2 cell extract: 0.39 - 200 µg/mL
Hela cell extract: 3.125 - 200 µg/mL
Store all components at 4°C. This kit is stable for at least 6 months from receipt. Unused microplate strips should be returned to the pouch containing the desiccant and resealed.
Tested applications
Sandwich ELISAmore details
Properties
Storage instructions
Please see Notes section
| Components | Identifier | 1 x 96 tests |
|---|---|---|
| 10X Blocking Buffer | 8209802 | 1 x 6ml |
| 10X HRP Label | 8206020 | 1 x 1ml |
| 10X HSP60 Detector Antibody | 8209557 | 1 x 1ml |
| 20X Buffer | 8203013 | 1 x 20ml |
| Extraction Buffer | 8201093 | 1 x 15ml |
| HSP60 Microplate | 8209558 | 1 unit |
| TMB Development Solution | 8209803 | 1 x 12ml |
Research areas
Signal Transduction >> Protein Trafficking >> Chaperones >> Heat Shock Proteins
Alternative names
- Chaperonin
- Heat shock 60kDa protein 1
Database links
- Entrez Gene: 3329 Human
- SwissProt: P10809 Human
Applications
Show applications key
Our Abpromise guarantee covers the use of ab124534 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
sELISA
HSP60 Human ELISA Kit images:
Sandwich ELISA - HSP60 Human ELISA Kit (ab124534)
Example standard curve of serially titrated HepG2 cell lysate in the working range of the assay.
Sandwich ELISA - HSP60 Human ELISA Kit (ab124534)
Example standard curve of serially titrated Hela cell lysate in the working range of the assay.
Western blot - HSP60 Human ELISA Kit (ab124534)
Detection of endogenous HSP60 levels in different cell lysates using Western blot. HSP60 antibody at 1µg/mL: HepG2 cell lysate (lane 2), HeLa cell lysate (lane 3), and HDFn lysate (lane 4), each were loaded at 15µg/lane.
Sandwich ELISA - HSP60 Human ELISA Kit (ab124534)
Detection of endogenous HSP60 levels in different cell lysates using HSP60 ELISA kit.
Sandwich ELISA - HSP60 Human ELISA Kit (ab124534)
Sensitivity of HSP60 ELISA kit is used to demonstrate the quantitative differentiation among different human cell lines.
Western blot - HSP60 Human ELISA Kit (ab124534)
Western blot using the HSP60 detector antibody at 1µg/mL demonstrates assay specificity. HepG2 cell lysate loaded at 15µg/lane.
Immunocytochemistry/ Immunofluorescence - HSP60 Human ELISA Kit (ab124534)
Analysis of HSP60 staining in HDFn cells by ICC: HSP60 primary antibody at 1 µg/mL (green).
Flow Cytometry - HSP60 Human ELISA Kit (ab124534)
Analysis of HSP60 staining in Hela cells by Flow: blue: 1µg/mL HSP60 antibody; red: an equal amount of an isotype control antibody.
Protocols
References for HSP60 Human ELISA Kit (ab124534)
ab124534 has not yet been referenced specifically in any publications.
Publishing research using ab124534? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"