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Do you know if this ELISA can be used with Neisseria meningitidis and Neisseria lactamica lysates? If not could you let me know if the antibodies in the kit could recognize Hsp70 of N.men or N.lac by an in silico prediction?
Asked on Oct 21 2013
Unfortunately, we have not tested this kit on Neisseria meningitidis and Neisseria lactamica lysates. I have compared the total human Hsp70 protein sequence with the Neisseria meningitidis and Neisseria lactamica sequences. Unfortunately, there is only 44% total homology between these species and therefore it is unlikely that this kit will work with your species.
Please also note that this kit will only detect induced Hsp70 and not constitutive Hsp70.
Elisa Thomas Abcam Scientific Support
Answered on Oct 21 2013