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ab19354 staining mouse embryonic brain tissue sections by IHC-P. Sections were PFA fixed and permeabilized in 0.3% Triton X-100 prior to blocking in 10% serum for 1 hour at 25°C. The primary antibody was diluted 1/400 and incubated with the sample for 16 hours at 4°C. A Cy3® conjugated donkey anti-goat antibody was used as the secondary.
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