![Immunofluorescence - HSV1 + HSV2 gB antibody [10B7] (ab6506) image](#ab6506_1.jpg)
![Western blot - HSV1 + HSV2 gB antibody [10B7] (ab6506) image](#ab6506_3.jpg)
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Products:Microbiology >> Organism >> Virus >> DNA Virus >> double stranded DNA Virus >> Herpes simplex
Overview
Product name
Anti-HSV1 + HSV2 gB antibody [10B7]
See all HSV1 + HSV2 gB products (2) ...
Description
Mouse monoclonal [10B7] to HSV1 + HSV2 gB
Tested applications
WB, ELISA, ICC/IFmore details
Immunogen
Herpes Virus
Positive control
African Green Monkey Kidney cultured cells(Vero), HSV-1 is strain MacIntyre, HSV-2 is strain G.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Phosphate buffered saline
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
10B7
Myeloma
NS1/1-Ag4-1
Isotype
IgG1
Light chain type
kappa
Research areas
Microbiology >> Organism >> Virus >> DNA Virus >> double stranded DNA Virus >> Herpes simplex
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Use under non reducing condition. (1/1600 (HSV2) - 1/12800 (HSV1).)
ELISA
ELISA: 1/102400ELISA: 1/102400
ICC/IF: Use at an assay dependent dilution. (1/6400 (HSV1) - 1/25600 (HSV2).)
Target
Relevance
There are two main types of Herpes Simplex Virus (HSV), 1 and 2. The primary difference between the two types is in where they typically establish latency in the body. HSV1 usually establishes latency in the trigeminal ganglion, a collection of nerve cells near the ear. From there, it tends to recur on the lower lip or face causing cold sores. HSV2 usually resides in the sacral ganglion at the base of the spine. From there, it recurs in the genital area but even this difference is not absolute as in rare cases it can infect any part of the body, including the eyes. Up to 30% of genital herpes are also caused by HSV1. Both HSV1 and 2 are highly contagious through contact.
Cellular localization
Virus envelope
Alternative names
- Envelope glycoprotein B antibody
- GB antibody
- Glycoprotein B antibody
see all
Anti-HSV1 + HSV2 gB antibody [10B7] images:
Immunofluorescence - HSV1 + HSV2 gB antibody [10B7] (ab6506)
![Immunofluorescence - HSV1 + HSV2 gB antibody [10B7] (ab6506)](/ps/datasheet/Images/6/ab6506/ab6506_1.jpg)
Green: immunofluorescence staining of HSV1 or HSV2 of Vero (African green monkey) cells ( HSV-1 is strain MacIntyre, HSV-2 is strain G) on slides, using ab6506 (1:100) (secondary anti-mouse-FITC). Red: counterstain in the conjugate preparation.
Western blot - HSV1 + HSV2 gB antibody [10B7] (ab6506)
![Western blot - HSV1 + HSV2 gB antibody [10B7] (ab6506)](/ps/datasheet/Images/6/ab6506/ab6506_3.jpg)
Lane 1 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/12800 dilution
Lane 2 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/1600 dilution
Lane 1 : Vero cells infected with HSV1
Lane 2 : Vero cells infected with HSV2
Performed under reducing conditions.
References for Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506)
This product has been referenced in:
- Goldwich Aet al. Herpes simplex virus type I (HSV-1) replicates in mature dendritic cells but can only be transferred in a cell-cell contact-dependent manner. J Leukoc Biol 89:973-9 (2011). WB.Read more (PubMed: 21427206) »
- Reszka NJet al. Construction and properties of a herpes simplex virus 2 dl5-29 vaccine candidate strain encoding an HSV-1 virion host shutoff protein. Vaccine 28:2754-62 (2010). WB.Read more (PubMed: 20117270) »
See all 2 publications for this product
Publishing research using ab6506? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"