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Immunofluorescence - HSV1 + HSV2 gB antibody [10B7] (ab6506)
![Immunofluorescence - HSV1 + HSV2 gB antibody [10B7] (ab6506)](/ps/datasheet/images/ab6506_1.jpg)
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Western blot - HSV1 + HSV2 gB antibody [10B7] (ab6506)
![Western blot - HSV1 + HSV2 gB antibody [10B7] (ab6506)](/ps/datasheet/images/ab6506_3.jpg)
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Product Name
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HSV1 + HSV2 gB antibody [10B7]
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See all HSV1 + HSV2 gB antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [10B7] to HSV1 + HSV2 gB
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Immunogen
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Herpes Virus
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Tested applications
(see key)
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ELISA, ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ELISA: 1/102400. IF (ICC): 1/6400 (HSV1) - 1/25600 (HSV2). Dilute antibody in PBS and incubate the slides for 1hr at 37C at high humidity. Wash in PBS, then incubate with secondary antibody for 1hr. WB: 1/1600 (HSV2) - 1/12800 (HSV1). Use under reducing conditions.
Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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African Green Monkey Kidney cultured cells(Vero), HSV-1 is strain MacIntyre, HSV-2 is strain G.
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Cellular localization
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Virus envelope
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Research areas
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Microbiology >> Organism >> Virus >> DNA Virus >> double stranded DNA Virus >> Herpes simplex
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Relevance
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There are two main types of Herpes Simplex Virus (HSV), 1 and 2. The primary difference between the two types is in where they typically establish latency in the body. HSV1 usually establishes latency in the trigeminal ganglion, a collection of nerve cells near the ear. From there, it tends to recur on the lower lip or face causing cold sores. HSV2 usually resides in the sacral ganglion at the base of the spine. From there, it recurs in the genital area but even this difference is not absolute as in rare cases it can infect any part of the body, including the eyes. Up to 30% of genital herpes are also caused by HSV1. Both HSV1 and 2 are highly contagious through contact.
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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10B7
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Myeloma
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NS1/1-Ag4-1
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Protein A purified
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Storage buffer
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Phosphate buffered saline
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HSV1 + HSV2 gB antibody [10B7] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for HSV1 + HSV2 gB antibody [10B7] images, references, products related to ab6506 and other tools.
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HSV1 + HSV2 gB antibody [10B7] images:
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Immunofluorescence - HSV1 + HSV2 gB antibody [10B7] (ab6506)
Green: immunofluorescence staining of HSV1 or HSV2 of Vero (African green monkey) cells ( HSV-1 is strain MacIntyre, HSV-2 is strain G) on slides, using ab6506 (1:100) (secondary anti-mouse-FITC).
Red: counterstain in the conjugate preparation.
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Western blot - HSV1 + HSV2 gB antibody [10B7] (ab6506)
Lane 1 : HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/12800 dilution Lane 2 : HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/1600 dilution
Lane 1 : Vero cells infected with HSV1 Lane 2 : Vero cells infected with HSV2
Performed under reducing conditions.
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Search PubMed (MEDLINE) for references to HSV1 + HSV2 gB
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HSV1 + HSV2 gB antibody [10B7] - more information
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Customer reviews (feedback) regarding HSV1 + HSV2 gB antibody [10B7] |
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HSV1 + HSV2 gB antibody [10B7] - related products:
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HSV1 + HSV2 gB antibody [10B7] - other tools:
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