HSV1 + HSV2 gB antibody [10B7] (ab6506)

Product Name 

HSV1 + HSV2 gB antibody [10B7]

Product type 

Primary antibodies

Description 

Mouse monoclonal [10B7] to HSV1 + HSV2 gB

Immunogen 

Herpes Virus

Tested applications 
(see key)

ELISA, ICC/IF, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: 1/102400.
IF (ICC): 1/6400 (HSV1) - 1/25600 (HSV2). Dilute antibody in PBS and incubate the slides for 1hr at 37C at high humidity. Wash in PBS, then incubate with secondary antibody for 1hr.
WB: 1/1600 (HSV2) - 1/12800 (HSV1). Use under reducing conditions.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

African Green Monkey Kidney cultured cells(Vero), HSV-1 is strain MacIntyre, HSV-2 is strain G.

Cellular localization 

Virus envelope

Research areas 

Microbiology >> Organism >> Virus >> DNA Virus >> double stranded DNA Virus >> Herpes simplex

Relevance 

There are two main types of Herpes Simplex Virus (HSV), 1 and 2. The primary difference between the two types is in where they typically establish latency in the body. HSV1 usually establishes latency in the trigeminal ganglion, a collection of nerve cells near the ear. From there, it tends to recur on the lower lip or face causing cold sores. HSV2 usually resides in the sacral ganglion at the base of the spine. From there, it recurs in the genital area but even this difference is not absolute as in rare cases it can infect any part of the body, including the eyes. Up to 30% of genital herpes are also caused by HSV1. Both HSV1 and 2 are highly contagious through contact.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

10B7

Myeloma 

NS1/1-Ag4-1

Isotype 

IgG1

Light chain type 

kappa

Purity 

Protein A purified

Storage buffer 

Phosphate buffered saline

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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