Anti-HURP antibody (ab70744)

Overview

• Product nameAnti-HURP antibodySee all HURP primary antibodies ...
• Description
  Rabbit polyclonal to HURP
• Tested applicationsWB, IP more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide corresponding to a region between residue 800 and the C-terminus (residue 846) of Human HURP (NP_055565.2).

• Positive controlHeLa and 293T whole cell lysates.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 0.1% BSA, Tris buffered saline
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Cell cycle
  • Cell division

  • Tags & Cell Markers
  • Subcellular Markers
  • Nucleus
  • Other Nuclear Bodies

  • Cell Biology
  • Cell Cycle
  • Cell Division
  • Spindle

Applications

Target

• FunctionPotential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.
• Tissue specificityAbundantly expressed in fetal liver. Expressed at lower levels in bone marrow, testis, colon, and placenta.
• Sequence similaritiesBelongs to the SAPAP family.
• Developmental stageElevated levels of expression detected in the G2/M phase of synchronized cultures of HeLa cells.
• Post-translational
  modificationsUbiquitinated, leading to its degradation.
  Phosphorylated upon DNA damage, probably by ATM or ATR. Decreased phosphorylation levels are associated with the differentiation of intestinal epithelial cells.
• Cellular localizationNucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Localizes to the spindle in mitotic cells. Colocalizes with CDH1 at sites of cell-cell contact in intestinal epithelial cells.

Target information above from: UniProt accession Q15398 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 9787 Human
  • SwissProt: Q15398 Human
  • Unigene: 77695 Human

• Alternative names
  DAP-5 antibodyDAP5 antibodyDiscs large homolog 7 antibody

  Disks large associated protein 5 antibodyDisks large associated protein DLG7 antibodyDisks large-associated protein 5 antibodyDisks large-associated protein DLG7 antibodyDLG 1 antibodyDLG 7 antibodyDLG1 antibodyDLG7 antibodyDLGAP5 antibodyDLGP5_HUMAN antibodyDrosophila discs large 1 tumor suppressor like antibodyHepatoma up regulated protein antibodyHepatoma up-regulated protein antibodyHepatoma upregulated protein antibodyHURP antibodyKIAA0008 antibody

  see all

Anti-HURP antibody images

• 

  Immunoprecipitation - HURP antibody (ab70744)
  Detection of HURP by Western Blot of Immunprecipitate. ab70744 at 1µg/ml staining HURP in HeLa whole cell lysate immunoprecipitated using ab70744 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
  Chemiluminescence with exposure time of 10 seconds.
• 

  Western blot - HURP antibody (ab70744)
  All lanes : Anti-HURP antibody (ab70744) at 0.04 µg/ml
  
  Lane 1 : HeLa whole cell lysate at 50 µg
  Lane 2 : HeLa whole cell lysate at 15 µg
  Lane 3 : HeLa whole cell lysate at 5 µg
  Lane 4 : 293T whole cell lysate at 50 µg
  
  
  Predicted band size : 94 kDa
  Observed band size : 120 kDa (why is the actual band size different from the predicted?)
  
  
  Exposure time : 3 minutes