Anti-HURP antibody (ab84509)

Overview

• Product nameAnti-HURP antibodySee all HURP primary antibodies ...
• Description
  Rabbit polyclonal to HURP
• Tested applicationsIHC-P, WB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide corresponding to a region between residues 200 and 250 of Human HURP (NP_055565.2).

• Positive controlHuman basal cell carcinoma and human seminoma tissues.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 0.1% BSA, Tris buffered saline
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Cell cycle
  • Cell division

  • Tags & Cell Markers
  • Subcellular Markers
  • Nucleus
  • Other Nuclear Bodies

  • Cell Biology
  • Cell Cycle
  • Cell Division
  • Spindle

Applications

Target

• FunctionPotential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.
• Tissue specificityAbundantly expressed in fetal liver. Expressed at lower levels in bone marrow, testis, colon, and placenta.
• Sequence similaritiesBelongs to the SAPAP family.
• Developmental stageElevated levels of expression detected in the G2/M phase of synchronized cultures of HeLa cells.
• Post-translational
  modificationsUbiquitinated, leading to its degradation.
  Phosphorylated upon DNA damage, probably by ATM or ATR. Decreased phosphorylation levels are associated with the differentiation of intestinal epithelial cells.
• Cellular localizationNucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Localizes to the spindle in mitotic cells. Colocalizes with CDH1 at sites of cell-cell contact in intestinal epithelial cells.

Target information above from: UniProt accession Q15398 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 9787 Human
  • SwissProt: Q15398 Human
  • Unigene: 77695 Human

• Alternative names
  DAP-5 antibodyDAP5 antibodyDiscs large homolog 7 antibody

  Disks large associated protein 5 antibodyDisks large associated protein DLG7 antibodyDisks large-associated protein 5 antibodyDisks large-associated protein DLG7 antibodyDLG 1 antibodyDLG 7 antibodyDLG1 antibodyDLG7 antibodyDLGAP5 antibodyDLGP5_HUMAN antibodyDrosophila discs large 1 tumor suppressor like antibodyHepatoma up regulated protein antibodyHepatoma up-regulated protein antibodyHepatoma upregulated protein antibodyHURP antibodyKIAA0008 antibody

  see all

Anti-HURP antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HURP antibody (ab84509)
  ab84509, at 1/250 dilution, staining HURP in formalin-fixed, paraffin-embedded human basal cell carcinoma by Immunohistochemistry, using DAB staining.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HURP antibody (ab84509)
  ab84509, at 1/250 dilution, staining HURP in formalin-fixed, paraffin-embedded human seminoma by Immunohistochemistry, using DAB staining.
• 

  Western blot - HURP antibody (ab84509)
  Anti-HURP antibody (ab84509) at 1/200 dilution + 25µg of WCE from U20S cells synchronized by thimidine arrest and release to enrich in mitotic cells (same on lane 1 and 2).