• Product name
  • Description
    Rabbit polyclonal to HURP
  • Tested applications
    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region between residues 200 and 250 of Human HURP (NP_055565.2).

  • Positive control
    • Human basal cell carcinoma and human seminoma tissues.



Our Abpromise guarantee covers the use of ab84509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
    WB: 1/200.

    Is unsuitable for ICC/IF.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.
    • Tissue specificity
      Abundantly expressed in fetal liver. Expressed at lower levels in bone marrow, testis, colon, and placenta.
    • Sequence similarities
      Belongs to the SAPAP family.
    • Developmental stage
      Elevated levels of expression detected in the G2/M phase of synchronized cultures of HeLa cells.
    • Post-translational
      Ubiquitinated, leading to its degradation.
      Phosphorylated upon DNA damage, probably by ATM or ATR. Decreased phosphorylation levels are associated with the differentiation of intestinal epithelial cells.
    • Cellular localization
      Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Localizes to the spindle in mitotic cells. Colocalizes with CDH1 at sites of cell-cell contact in intestinal epithelial cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • DAP-5 antibody
      • DAP5 antibody
      • Discs large homolog 7 antibody
      • Disks large associated protein 5 antibody
      • Disks large associated protein DLG7 antibody
      • Disks large-associated protein 5 antibody
      • Disks large-associated protein DLG7 antibody
      • DLG 1 antibody
      • DLG 7 antibody
      • DLG1 antibody
      • DLG7 antibody
      • DLGAP5 antibody
      • DLGP5_HUMAN antibody
      • Drosophila discs large 1 tumor suppressor like antibody
      • Hepatoma up regulated protein antibody
      • Hepatoma up-regulated protein antibody
      • Hepatoma upregulated protein antibody
      • HURP antibody
      • KIAA0008 antibody
      see all

    Anti-HURP antibody images

    • ab84509, at 1/250 dilution, staining HURP in formalin-fixed, paraffin-embedded human basal cell carcinoma by Immunohistochemistry, using DAB staining.
    • ab84509, at 1/250 dilution, staining HURP in formalin-fixed, paraffin-embedded human seminoma by Immunohistochemistry, using DAB staining.
    • Anti-HURP antibody (ab84509) at 1/200 dilution + 25µg of WCE from U20S cells synchronized by thimidine arrest and release to enrich in mitotic cells (same on lane 1 and 2).

    References for Anti-HURP antibody (ab84509)

    This product has been referenced in:
    • Ma MP  et al. Sorting nexin 9 recruits clathrin heavy chain to the mitotic spindle for chromosome alignment and segregation. PLoS One 8:e68387 (2013). Read more (PubMed: 23861900) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab84509.
    Please use the links above to contact us or submit feedback about this product.


    Sign up