The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
Is unsuitable for ICC/IF.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPotential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.
Tissue specificityAbundantly expressed in fetal liver. Expressed at lower levels in bone marrow, testis, colon, and placenta.
Sequence similaritiesBelongs to the SAPAP family.
Developmental stageElevated levels of expression detected in the G2/M phase of synchronized cultures of HeLa cells.
Post-translational modificationsUbiquitinated, leading to its degradation. Phosphorylated upon DNA damage, probably by ATM or ATR. Decreased phosphorylation levels are associated with the differentiation of intestinal epithelial cells.
Cellular localizationNucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Localizes to the spindle in mitotic cells. Colocalizes with CDH1 at sites of cell-cell contact in intestinal epithelial cells.