Overview

  • Product name
  • Description
    Rabbit polyclonal to HURP
  • Tested applications
    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region between residues 200 and 250 of Human HURP (NP_055565.2).

  • Positive control
    • Human basal cell carcinoma and human seminoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab84509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
  • Application notes
    IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
    WB: 1/200.

    Is unsuitable for ICC/IF.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.
    • Tissue specificity
      Abundantly expressed in fetal liver. Expressed at lower levels in bone marrow, testis, colon, and placenta.
    • Sequence similarities
      Belongs to the SAPAP family.
    • Developmental stage
      Elevated levels of expression detected in the G2/M phase of synchronized cultures of HeLa cells.
    • Post-translational
      modifications
      Ubiquitinated, leading to its degradation.
      Phosphorylated upon DNA damage, probably by ATM or ATR. Decreased phosphorylation levels are associated with the differentiation of intestinal epithelial cells.
    • Cellular localization
      Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Localizes to the spindle in mitotic cells. Colocalizes with CDH1 at sites of cell-cell contact in intestinal epithelial cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • DAP-5 antibody
      • DAP5 antibody
      • Discs large homolog 7 antibody
      • Disks large associated protein 5 antibody
      • Disks large associated protein DLG7 antibody
      • Disks large-associated protein 5 antibody
      • Disks large-associated protein DLG7 antibody
      • DLG 1 antibody
      • DLG 7 antibody
      • DLG1 antibody
      • DLG7 antibody
      • DLGAP5 antibody
      • DLGP5_HUMAN antibody
      • Drosophila discs large 1 tumor suppressor like antibody
      • Hepatoma up regulated protein antibody
      • Hepatoma up-regulated protein antibody
      • Hepatoma upregulated protein antibody
      • HURP antibody
      • KIAA0008 antibody
      see all

    Images

    • ab84509, at 1/250 dilution, staining HURP in formalin-fixed, paraffin-embedded human basal cell carcinoma by Immunohistochemistry, using DAB staining.
    • ab84509, at 1/250 dilution, staining HURP in formalin-fixed, paraffin-embedded human seminoma by Immunohistochemistry, using DAB staining.
    • Anti-HURP antibody (ab84509) at 1/200 dilution + 25µg of WCE from U20S cells synchronized by thimidine arrest and release to enrich in mitotic cells (same on lane 1 and 2).

    References

    This product has been referenced in:
    • Espinoza I  et al. Hypoxia on the Expression of Hepatoma Upregulated Protein in Prostate Cancer Cells. Front Oncol 6:144 (2016). IHC . Read more (PubMed: 27379206) »
    • Ma MP  et al. Sorting nexin 9 recruits clathrin heavy chain to the mitotic spindle for chromosome alignment and segregation. PLoS One 8:e68387 (2013). Read more (PubMed: 23861900) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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