HVEM Human ELISA Kit (ab113335)
- Product nameHVEM Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 30 pg/ml
- Range32.77 pg/ml - 8000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 85.34 69% - 98% Serum 90.17 81% - 97% Plasma 90.83 73% - 112%
- Species reactivityReacts with: Human
- Product overview
ab113335 Human HVEM (herpes virus entry mediator) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human HVEM in serum, plasma, cell culture supernatants. This assay employs an antibody specific for human HVEM coated on a 96-well plate. Standards and samples are pipetted into the wells and HVEM present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human HVEM antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HVEM bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 200X HRP-Streptavidin Concentrate Item G 1 x 200µl 20X Wash Buffer Concentrate Item B 1 x 25ml Assay Diluent B Item E 1 x 15ml Assay Diluent C Item L 1 x 30ml Detection Antibody HVEM Item F 2 vials HVEM Microplate (12 strips x 8 wells) coated with anti-Human HVEM Item A 1 x 96 tests HVEM Standard Human (recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
- CD270Herpes virus entry mediator AHerpesvirus entry mediator A
- HveATNFRSF14TNR14_HUMANTR2Tumor necrosis factor receptor superfamily member 14Tumor necrosis factor receptor-like 2
Our Abpromise guarantee covers the use of ab113335 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for HVEM Human ELISA Kit (ab113335)
ab113335 has not yet been referenced specifically in any publications.