Human Haptoglobin ELISA Kit (ab108856)
Key features and details
- Sensitivity: 57 ng/ml
- Range: 0.078 µg/ml - 20 µg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
-
Product name
Human Haptoglobin ELISA Kit
See all Haptoglobin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 6.1% Inter-assay Sample n Mean SD CV% Overall 10.54% -
Sample type
Serum, Plasma -
Assay type
Competitive -
Sensitivity
= 57 ng/ml -
Range
0.078 µg/ml - 20 µg/ml -
Recovery
= 90 %
-
Assay time
2h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Haptoglobin in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Haptoglobin in plasma and serum.
A Haptoglobin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Haptoglobin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Haptoglobin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
-
Platform
Microplate
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Biotinylated Human Haptoglobin (Lyophilized) 1 vial 20X Wash Buffer Concentrate 1 x 30ml Chromogen Substrate 1 x 7ml Haptoglobin Microplate (12 x 8 well strips) 1 unit Haptoglobin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
Research areas
-
Function
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Anhaptoglobinemia -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- Binding peptide
- BP
- Haptoglobin alpha chain
see all -
Database links
- Entrez Gene: 3240 Human
- Omim: 140100 Human
- SwissProt: P00738 Human
- Unigene: 513711 Human
- Unigene: 702099 Human
Images
Datasheets and documents
-
SDS download
-
Datasheet download
References (11)
ab108856 has been referenced in 11 publications.
- Muñoz-San Martín C et al. First bioelectronic immunoplatform for quantitative secretomic analysis of total and metastasis-driven glycosylated haptoglobin. Anal Bioanal Chem 415:2045-2057 (2023). PubMed: 36344668
- Macarlupú JL et al. Sub-maximal aerobic exercise training reduces haematocrit and ameliorates symptoms in Andean highlanders with chronic mountain sickness. Exp Physiol 106:2198-2209 (2021). PubMed: 34555237
- Paul S et al. Plasma protein(s)-based conceptual diagnostic tool for assessing high-altitude acclimation in humans. Funct Integr Genomics 20:191-200 (2020). PubMed: 31444657
- Tran TTT et al. APOE genotype influences the gut microbiome structure and function in humans and mice: relevance for Alzheimer's disease pathophysiology. FASEB J N/A:fj201900071R (2019). PubMed: 30958695
- Lv LL et al. Effect of haptoglobin on the treatment of chronic hepatitis B with interferon. Exp Ther Med 18:1417-1425 (2019). PubMed: 31316628