Anti-Haptoglobin antibody (ab131146)
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
- Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab131146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||IHC-P: Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
- FunctionAs a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
- Tissue specificityExpressed by the liver and secreted in plasma.
- Involvement in diseaseAnhaptoglobinemia (AHP) [MIM:614081]: A condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin. Congenital haptoglobin deficiency is a risk factor for anaphylactic non-hemolytic transfusion reactions. Note=The disease is caused by mutations affecting the gene represented in this entry.
- Sequence similaritiesBelongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains.
- Cellular localizationSecreted.
- Binding peptide antibodyBP antibodyHaptoglobin alpha chain antibody
- Haptoglobin alpha(1S) beta antibodyHaptoglobin alpha(2FS) beta antibodyHaptoglobin beta chain antibodyHaptoglobin, alpha polypeptide antibodyHaptoglobin, beta polypeptide antibodyHP antibodyHP2 ALPHA2 antibodyHP2ALPHA2 antibodyHPA1S antibodyHPT antibodyHPT_HUMAN antibodyMGC111141 antibodyZonulin antibody
Anti-Haptoglobin antibody images
Immunohistochemical analysis of paraffin embedded Human liver tissue labelling Haptoglobin with ab131146 at 2.5 µg/ml.
References for Anti-Haptoglobin antibody (ab131146)
ab131146 has not yet been referenced specifically in any publications.